<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:a12c9650-c665-4945-913e-9e9f011ed114,g:485e89bc-d27f-4026-942d-5c7ac04342fe-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://tarsusrx.com/role/CondensedStatementsofOperationsandComprehensiveLoss" xlink:type="simple" xlink:href="tars-20240331.xsd#CondensedStatementsofOperationsandComprehensiveLoss"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/CondensedStatementsofOperationsandComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_bdf1efc1-d777-4973-bf55-abcaf8cfd8c3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_StatementTable_bdf1efc1-d777-4973-bf55-abcaf8cfd8c3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_35bf6bfe-9605-4f75-98ed-fe386bbade00" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_bdf1efc1-d777-4973-bf55-abcaf8cfd8c3" xlink:to="loc_srt_ProductOrServiceAxis_35bf6bfe-9605-4f75-98ed-fe386bbade00" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_35bf6bfe-9605-4f75-98ed-fe386bbade00_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_35bf6bfe-9605-4f75-98ed-fe386bbade00" xlink:to="loc_srt_ProductsAndServicesDomain_35bf6bfe-9605-4f75-98ed-fe386bbade00_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_a119080e-8a30-43cf-843d-75053403fd59" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_35bf6bfe-9605-4f75-98ed-fe386bbade00" xlink:to="loc_srt_ProductsAndServicesDomain_a119080e-8a30-43cf-843d-75053403fd59" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductMember_e4048cec-2c98-47bf-9e7d-a19aaf31de58" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a119080e-8a30-43cf-843d-75053403fd59" xlink:to="loc_us-gaap_ProductMember_e4048cec-2c98-47bf-9e7d-a19aaf31de58" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LicenseAndCollaborationMember_6d5ff05c-c041-4b35-982d-24f3b78bcee3" xlink:href="tars-20240331.xsd#tars_LicenseAndCollaborationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a119080e-8a30-43cf-843d-75053403fd59" xlink:to="loc_tars_LicenseAndCollaborationMember_6d5ff05c-c041-4b35-982d-24f3b78bcee3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_efcab350-70c8-4ee8-8225-44ecce675371" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenuesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_RevenuesAbstract_efcab350-70c8-4ee8-8225-44ecce675371" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_59bf7f1e-83c7-4900-877b-3f8bc24db5fd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_efcab350-70c8-4ee8-8225-44ecce675371" xlink:to="loc_us-gaap_Revenues_59bf7f1e-83c7-4900-877b-3f8bc24db5fd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpensesAbstract_5b7599c4-f62c-46c3-ab17-bb55459c8442" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingExpensesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_OperatingExpensesAbstract_5b7599c4-f62c-46c3-ab17-bb55459c8442" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_54a8d63a-db34-4ff0-8202-d79e0a2729ae" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_5b7599c4-f62c-46c3-ab17-bb55459c8442" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_54a8d63a-db34-4ff0-8202-d79e0a2729ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_ea397d1d-db8e-480d-b705-b137d8b88431" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_5b7599c4-f62c-46c3-ab17-bb55459c8442" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_ea397d1d-db8e-480d-b705-b137d8b88431" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_98cac7ad-22c9-4d03-ac71-dc8a552aea88" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_5b7599c4-f62c-46c3-ab17-bb55459c8442" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_98cac7ad-22c9-4d03-ac71-dc8a552aea88" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_c3b32644-5c1c-439d-b39d-f3102d186f56" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostsAndExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_5b7599c4-f62c-46c3-ab17-bb55459c8442" xlink:to="loc_us-gaap_CostsAndExpenses_c3b32644-5c1c-439d-b39d-f3102d186f56" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_73e924d8-c7bd-4447-be08-bebf022ab4e7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_OperatingIncomeLoss_73e924d8-c7bd-4447-be08-bebf022ab4e7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpenseAbstract_e81589a9-1672-474c-8041-1027f4a57e92" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NonoperatingIncomeExpenseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_NonoperatingIncomeExpenseAbstract_e81589a9-1672-474c-8041-1027f4a57e92" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeOther_ffebb73c-2583-4c32-af8d-3c7f80fc7243" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestIncomeOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_e81589a9-1672-474c-8041-1027f4a57e92" xlink:to="loc_us-gaap_InterestIncomeOther_ffebb73c-2583-4c32-af8d-3c7f80fc7243" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_98585ebc-8fce-4c72-ad85-dee23903d7d6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_e81589a9-1672-474c-8041-1027f4a57e92" xlink:to="loc_us-gaap_InterestExpense_98585ebc-8fce-4c72-ad85-dee23903d7d6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_c46d1826-920d-465a-b7ef-b5660e39945c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_e81589a9-1672-474c-8041-1027f4a57e92" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_c46d1826-920d-465a-b7ef-b5660e39945c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiUnrealizedGainLoss_eb637956-5f2e-4873-b2cd-88f126fbfb8c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesFvNiUnrealizedGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_e81589a9-1672-474c-8041-1027f4a57e92" xlink:to="loc_us-gaap_EquitySecuritiesFvNiUnrealizedGainLoss_eb637956-5f2e-4873-b2cd-88f126fbfb8c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmbeddedDerivativeGainLossOnEmbeddedDerivativeNet_323ee1ad-a383-47a5-9efc-b8e33e243f24" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmbeddedDerivativeGainLossOnEmbeddedDerivativeNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_e81589a9-1672-474c-8041-1027f4a57e92" xlink:to="loc_us-gaap_EmbeddedDerivativeGainLossOnEmbeddedDerivativeNet_323ee1ad-a383-47a5-9efc-b8e33e243f24" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_7467d3fa-145f-4e5e-96a6-444aef3208c4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_e81589a9-1672-474c-8041-1027f4a57e92" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_7467d3fa-145f-4e5e-96a6-444aef3208c4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_ed9a86e5-8ffd-4a48-bda9-1131e61ebeca" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_NetIncomeLoss_ed9a86e5-8ffd-4a48-bda9-1131e61ebeca" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract_ba244957-0842-4359-8ad6-6d4410421558" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract_ba244957-0842-4359-8ad6-6d4410421558" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax_0ddf9e0d-ce79-4bb6-9e65-4e0381ebed8f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract_ba244957-0842-4359-8ad6-6d4410421558" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax_0ddf9e0d-ce79-4bb6-9e65-4e0381ebed8f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_06437683-f6f6-4334-8512-214201bbe413" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract_ba244957-0842-4359-8ad6-6d4410421558" xlink:to="loc_us-gaap_ComprehensiveIncomeNetOfTax_06437683-f6f6-4334-8512-214201bbe413" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareAbstract_d5483e6a-3bbb-4789-90f5-932edcc53b8b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EarningsPerShareAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_EarningsPerShareAbstract_d5483e6a-3bbb-4789-90f5-932edcc53b8b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_07742e81-5ebd-4725-b788-02717209e62e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_d5483e6a-3bbb-4789-90f5-932edcc53b8b" xlink:to="loc_us-gaap_EarningsPerShareBasic_07742e81-5ebd-4725-b788-02717209e62e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_eaeb6dc7-5fff-4791-9085-6f60b6cb4aaf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_d5483e6a-3bbb-4789-90f5-932edcc53b8b" xlink:to="loc_us-gaap_EarningsPerShareDiluted_eaeb6dc7-5fff-4791-9085-6f60b6cb4aaf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_ae644d1d-6092-4ce2-87c0-e1b57f9c8600" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_eca3410d-c7b2-4579-b9a9-3ba31fe9922d" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_ae644d1d-6092-4ce2-87c0-e1b57f9c8600" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_98f02993-20e5-4752-8526-d6d60ac888d4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_ae644d1d-6092-4ce2-87c0-e1b57f9c8600" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_98f02993-20e5-4752-8526-d6d60ac888d4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_e5a4b171-d140-472b-8f84-fa007431ef54" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_ae644d1d-6092-4ce2-87c0-e1b57f9c8600" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_e5a4b171-d140-472b-8f84-fa007431ef54" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/CondensedStatementsofStockholdersEquity" xlink:type="simple" xlink:href="tars-20240331.xsd#CondensedStatementsofStockholdersEquity"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/CondensedStatementsofStockholdersEquity" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_710e89fe-953c-48d5-a5e3-3d6c784e2d40" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_ddb0389c-dbb0-4bb2-b91f-c4fc7992c1de" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_710e89fe-953c-48d5-a5e3-3d6c784e2d40" xlink:to="loc_us-gaap_StatementTable_ddb0389c-dbb0-4bb2-b91f-c4fc7992c1de" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_ebee4c5f-801d-4fa6-b6d7-b1ab014c7ba8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_ddb0389c-dbb0-4bb2-b91f-c4fc7992c1de" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_ebee4c5f-801d-4fa6-b6d7-b1ab014c7ba8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_ebee4c5f-801d-4fa6-b6d7-b1ab014c7ba8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_ebee4c5f-801d-4fa6-b6d7-b1ab014c7ba8" xlink:to="loc_us-gaap_EquityComponentDomain_ebee4c5f-801d-4fa6-b6d7-b1ab014c7ba8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_652eb08a-3488-4e8f-a2bf-4523f8008194" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_ebee4c5f-801d-4fa6-b6d7-b1ab014c7ba8" xlink:to="loc_us-gaap_EquityComponentDomain_652eb08a-3488-4e8f-a2bf-4523f8008194" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_8bcd94b6-d2e4-4391-96ef-b6e5df75cdc0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_652eb08a-3488-4e8f-a2bf-4523f8008194" xlink:to="loc_us-gaap_CommonStockMember_8bcd94b6-d2e4-4391-96ef-b6e5df75cdc0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_e35efe1b-f805-4d62-9c13-d77a46e87ee2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_652eb08a-3488-4e8f-a2bf-4523f8008194" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_e35efe1b-f805-4d62-9c13-d77a46e87ee2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_4ba20b46-302f-4a28-92fb-6ad0d7accbd8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_652eb08a-3488-4e8f-a2bf-4523f8008194" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_4ba20b46-302f-4a28-92fb-6ad0d7accbd8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_f02554b0-6cef-488f-8001-40de447034fa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_652eb08a-3488-4e8f-a2bf-4523f8008194" xlink:to="loc_us-gaap_RetainedEarningsMember_f02554b0-6cef-488f-8001-40de447034fa" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_710e89fe-953c-48d5-a5e3-3d6c784e2d40" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_1a52b644-9ec5-470a-a7fc-54288f7ef8fa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_1a52b644-9ec5-470a-a7fc-54288f7ef8fa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_857674f6-d024-424d-adcf-49246c35642f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_StockholdersEquity_857674f6-d024-424d-adcf-49246c35642f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_33504102-f7e4-4fd6-8817-e627bfc34ec8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_NetIncomeLoss_33504102-f7e4-4fd6-8817-e627bfc34ec8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_04c49a50-375d-4747-ab5f-fc8aa7871d3c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_04c49a50-375d-4747-ab5f-fc8aa7871d3c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_88bdd60d-e53f-408e-8339-6516ec82bc12" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_88bdd60d-e53f-408e-8339-6516ec82bc12" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_7e36dc06-6feb-452c-b2f1-a01d4a183637" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_7e36dc06-6feb-452c-b2f1-a01d4a183637" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued_3e3f3fa1-c7b4-455b-a6bf-dfe16579a7cf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued_3e3f3fa1-c7b4-455b-a6bf-dfe16579a7cf" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_02276acf-3960-4efc-9ef6-c7cc33abeca4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_02276acf-3960-4efc-9ef6-c7cc33abeca4" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_3c2a1ca2-1e97-4f49-a0bc-8a7e17081742" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_3c2a1ca2-1e97-4f49-a0bc-8a7e17081742" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_c031a4e2-3512-483f-91eb-c75c15b91f79" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_c031a4e2-3512-483f-91eb-c75c15b91f79" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_7a9d8e7d-0211-4582-90cb-fb31db209d78" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_96b1ab5e-1980-4d85-9f60-a10b183db578" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_7a9d8e7d-0211-4582-90cb-fb31db209d78" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_4313f84f-ef85-498b-9c5e-329e5248ec15" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_1aac1091-db35-431f-afb5-752d73f4edca" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/DescriptionofBusinessandPresentationofFinancialStatementsDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#DescriptionofBusinessandPresentationofFinancialStatementsDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/DescriptionofBusinessandPresentationofFinancialStatementsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_5ab02934-9b78-4f01-a545-f79b2f82bc87" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_5ab02934-9b78-4f01-a545-f79b2f82bc87" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_b98af327-2bfc-4e4a-8654-b5f398d32c5c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_5ab02934-9b78-4f01-a545-f79b2f82bc87" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_b98af327-2bfc-4e4a-8654-b5f398d32c5c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_b98af327-2bfc-4e4a-8654-b5f398d32c5c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_b98af327-2bfc-4e4a-8654-b5f398d32c5c" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_b98af327-2bfc-4e4a-8654-b5f398d32c5c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_6ae0b63e-3ff6-4799-a6df-e45b026e4162" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_b98af327-2bfc-4e4a-8654-b5f398d32c5c" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_6ae0b63e-3ff6-4799-a6df-e45b026e4162" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_FollowOnPublicOfferingMember_a6af0af1-cbe3-4a83-a616-b8612974e090" xlink:href="tars-20240331.xsd#tars_FollowOnPublicOfferingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_6ae0b63e-3ff6-4799-a6df-e45b026e4162" xlink:to="loc_tars_FollowOnPublicOfferingMember_a6af0af1-cbe3-4a83-a616-b8612974e090" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OverAllotmentOptionMember_daec0e02-33b2-4d66-83ee-2cb188d0af01" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OverAllotmentOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_6ae0b63e-3ff6-4799-a6df-e45b026e4162" xlink:to="loc_us-gaap_OverAllotmentOptionMember_daec0e02-33b2-4d66-83ee-2cb188d0af01" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_A2023ShelfRegistrationStatementMember_b1a74bcb-f407-4e92-aef3-e057b014e67e" xlink:href="tars-20240331.xsd#tars_A2023ShelfRegistrationStatementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_6ae0b63e-3ff6-4799-a6df-e45b026e4162" xlink:to="loc_tars_A2023ShelfRegistrationStatementMember_b1a74bcb-f407-4e92-aef3-e057b014e67e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_A2023ATMProspectusMember_2c3ec226-8247-42b9-88f1-1029595c5c4d" xlink:href="tars-20240331.xsd#tars_A2023ATMProspectusMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_6ae0b63e-3ff6-4799-a6df-e45b026e4162" xlink:to="loc_tars_A2023ATMProspectusMember_2c3ec226-8247-42b9-88f1-1029595c5c4d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_3253f87f-55b8-4bdc-82ca-ee4c833758b4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_3253f87f-55b8-4bdc-82ca-ee4c833758b4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_26d59f18-a261-4673-88fd-88813d3043f7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_26d59f18-a261-4673-88fd-88813d3043f7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ClassOfWarrantOrRightReservedForFutureIssuance_f2e7df87-1dc1-4399-a251-ac3db984241a" xlink:href="tars-20240331.xsd#tars_ClassOfWarrantOrRightReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_tars_ClassOfWarrantOrRightReservedForFutureIssuance_f2e7df87-1dc1-4399-a251-ac3db984241a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockPricePerShare_4f6794f7-55eb-4569-9bdc-5ed2012482dd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockPricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_SaleOfStockPricePerShare_4f6794f7-55eb-4569-9bdc-5ed2012482dd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ClassOfWarrantOrRightSalePrice_5269ac62-1aa3-4a84-98c1-1343047cbf31" xlink:href="tars-20240331.xsd#tars_ClassOfWarrantOrRightSalePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_tars_ClassOfWarrantOrRightSalePrice_5269ac62-1aa3-4a84-98c1-1343047cbf31" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_9c4eab9a-b821-4261-8eac-408659a65bca" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_9c4eab9a-b821-4261-8eac-408659a65bca" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_0d1d641d-f5f8-4319-a31d-0e5d176cac8c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockConsiderationReceivedOnTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_0d1d641d-f5f8-4319-a31d-0e5d176cac8c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_SaleOfStockSalesAgentFeePercentageOfGrossProceeds_3eafdde1-2e3c-4cb4-b663-138a6bfad229" xlink:href="tars-20240331.xsd#tars_SaleOfStockSalesAgentFeePercentageOfGrossProceeds"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_tars_SaleOfStockSalesAgentFeePercentageOfGrossProceeds_3eafdde1-2e3c-4cb4-b663-138a6bfad229" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_SaleOfStockConsiderationAmountAuthorized_b9242131-0bd7-4d3d-8469-535eefeb1208" xlink:href="tars-20240331.xsd#tars_SaleOfStockConsiderationAmountAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_tars_SaleOfStockConsiderationAmountAuthorized_b9242131-0bd7-4d3d-8469-535eefeb1208" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_754a4242-120c-403b-bd4e-005393be5bb3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_754a4242-120c-403b-bd4e-005393be5bb3" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_71749c40-166c-43bc-8df7-8183cb11f301" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_NumberOfReportableSegments_71749c40-166c-43bc-8df7-8183cb11f301" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_bbe7e1ca-d429-4226-876a-d927ca430820" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NumberOfOperatingSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f33fe9ef-f470-45ab-82e3-8924c72adb43" xlink:to="loc_us-gaap_NumberOfOperatingSegments_bbe7e1ca-d429-4226-876a-d927ca430820" xlink:type="arc" order="11"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/SummaryofSignificantAccountingPoliciesandUseofEstimatesIntangibleAssetsNetDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#SummaryofSignificantAccountingPoliciesandUseofEstimatesIntangibleAssetsNetDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/SummaryofSignificantAccountingPoliciesandUseofEstimatesIntangibleAssetsNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_0d0df111-c258-482d-b2f4-619c26a7d21f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_02df76ca-73f4-408a-8683-08da5bd7d7d2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_0d0df111-c258-482d-b2f4-619c26a7d21f" xlink:to="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_02df76ca-73f4-408a-8683-08da5bd7d7d2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis_b445b2c0-40fd-45a8-b608-96ca5922b082" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_02df76ca-73f4-408a-8683-08da5bd7d7d2" xlink:to="loc_us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis_b445b2c0-40fd-45a8-b608-96ca5922b082" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_b445b2c0-40fd-45a8-b608-96ca5922b082_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongLivedAssetsHeldForSaleNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis_b445b2c0-40fd-45a8-b608-96ca5922b082" xlink:to="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_b445b2c0-40fd-45a8-b608-96ca5922b082_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_d9c785fc-a52e-447a-9af9-a98fd9bfc07c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongLivedAssetsHeldForSaleNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis_b445b2c0-40fd-45a8-b608-96ca5922b082" xlink:to="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_d9c785fc-a52e-447a-9af9-a98fd9bfc07c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_IntangibleAssetsMilestonePaymentMember_e5f3fb5d-a141-468e-981b-24f543af9666" xlink:href="tars-20240331.xsd#tars_IntangibleAssetsMilestonePaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_d9c785fc-a52e-447a-9af9-a98fd9bfc07c" xlink:to="loc_tars_IntangibleAssetsMilestonePaymentMember_e5f3fb5d-a141-468e-981b-24f543af9666" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_490d22fc-3d92-413f-a90e-669041a0f47b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_0d0df111-c258-482d-b2f4-619c26a7d21f" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_490d22fc-3d92-413f-a90e-669041a0f47b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_e6087b6d-b774-4564-86d1-401e55917aaf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_0d0df111-c258-482d-b2f4-619c26a7d21f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_e6087b6d-b774-4564-86d1-401e55917aaf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill_09054900-39da-4b4e-951f-9dcb756d1758" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_0d0df111-c258-482d-b2f4-619c26a7d21f" xlink:to="loc_us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill_09054900-39da-4b4e-951f-9dcb756d1758" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/SummaryofSignificantAccountingPoliciesandUseofEstimatesDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#SummaryofSignificantAccountingPoliciesandUseofEstimatesDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/SummaryofSignificantAccountingPoliciesandUseofEstimatesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b2996b4c-eaea-4f32-a5b9-978a6f2157c1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b2996b4c-eaea-4f32-a5b9-978a6f2157c1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_ccc91039-f3e4-4a53-8d69-8370063beae9" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b2996b4c-eaea-4f32-a5b9-978a6f2157c1" xlink:to="loc_srt_RangeAxis_ccc91039-f3e4-4a53-8d69-8370063beae9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ccc91039-f3e4-4a53-8d69-8370063beae9_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_ccc91039-f3e4-4a53-8d69-8370063beae9" xlink:to="loc_srt_RangeMember_ccc91039-f3e4-4a53-8d69-8370063beae9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_5627c355-e518-48de-89f0-7e8fb83c3e53" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_ccc91039-f3e4-4a53-8d69-8370063beae9" xlink:to="loc_srt_RangeMember_5627c355-e518-48de-89f0-7e8fb83c3e53" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_a84fe3c9-84a3-4d37-94c9-4160ad777e99" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5627c355-e518-48de-89f0-7e8fb83c3e53" xlink:to="loc_srt_MinimumMember_a84fe3c9-84a3-4d37-94c9-4160ad777e99" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_d5c2c79c-0804-4c3e-8a7d-1cf264cecdde" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5627c355-e518-48de-89f0-7e8fb83c3e53" xlink:to="loc_srt_MaximumMember_d5c2c79c-0804-4c3e-8a7d-1cf264cecdde" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_b3aa0c5c-2bf1-44b9-8c3f-8666061c60f1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b2996b4c-eaea-4f32-a5b9-978a6f2157c1" xlink:to="loc_us-gaap_PlanNameAxis_b3aa0c5c-2bf1-44b9-8c3f-8666061c60f1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_b3aa0c5c-2bf1-44b9-8c3f-8666061c60f1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_b3aa0c5c-2bf1-44b9-8c3f-8666061c60f1" xlink:to="loc_us-gaap_PlanNameDomain_b3aa0c5c-2bf1-44b9-8c3f-8666061c60f1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_e922fce4-539f-4f3f-b6a9-28c3bd306f16" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_b3aa0c5c-2bf1-44b9-8c3f-8666061c60f1" xlink:to="loc_us-gaap_PlanNameDomain_e922fce4-539f-4f3f-b6a9-28c3bd306f16" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_EquityIncentivePlan2016Member_4a069717-2008-44a2-9bba-425f92550fd1" xlink:href="tars-20240331.xsd#tars_EquityIncentivePlan2016Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_e922fce4-539f-4f3f-b6a9-28c3bd306f16" xlink:to="loc_tars_EquityIncentivePlan2016Member_4a069717-2008-44a2-9bba-425f92550fd1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_88c036b9-b05d-41c3-87b1-8839b2b9b577" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b2996b4c-eaea-4f32-a5b9-978a6f2157c1" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_88c036b9-b05d-41c3-87b1-8839b2b9b577" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_88c036b9-b05d-41c3-87b1-8839b2b9b577_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_88c036b9-b05d-41c3-87b1-8839b2b9b577" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_88c036b9-b05d-41c3-87b1-8839b2b9b577_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_d5081e99-8582-4e2b-aa3a-07a53e3438e4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_88c036b9-b05d-41c3-87b1-8839b2b9b577" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_d5081e99-8582-4e2b-aa3a-07a53e3438e4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesRevenueNetMember_0056e173-da7a-4add-b1f9-8334f5be2021" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SalesRevenueNetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_d5081e99-8582-4e2b-aa3a-07a53e3438e4" xlink:to="loc_us-gaap_SalesRevenueNetMember_0056e173-da7a-4add-b1f9-8334f5be2021" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableMember_1c9d14ff-99b7-4267-b109-13f423b2826d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_d5081e99-8582-4e2b-aa3a-07a53e3438e4" xlink:to="loc_us-gaap_AccountsReceivableMember_1c9d14ff-99b7-4267-b109-13f423b2826d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_1e586146-8b45-4d20-9191-6af4b1b4f5ed" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b2996b4c-eaea-4f32-a5b9-978a6f2157c1" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_1e586146-8b45-4d20-9191-6af4b1b4f5ed" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_1e586146-8b45-4d20-9191-6af4b1b4f5ed_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_1e586146-8b45-4d20-9191-6af4b1b4f5ed" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_1e586146-8b45-4d20-9191-6af4b1b4f5ed_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_23162c4b-bc2b-4aac-b0e3-9bbb07567272" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_1e586146-8b45-4d20-9191-6af4b1b4f5ed" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_23162c4b-bc2b-4aac-b0e3-9bbb07567272" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_211ac48a-65de-4e69-8556-d7edeae63c69" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CustomerConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_23162c4b-bc2b-4aac-b0e3-9bbb07567272" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_211ac48a-65de-4e69-8556-d7edeae63c69" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_08c3d3f7-8c42-4f20-83d9-46fdb520a61f" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b2996b4c-eaea-4f32-a5b9-978a6f2157c1" xlink:to="loc_srt_MajorCustomersAxis_08c3d3f7-8c42-4f20-83d9-46fdb520a61f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_08c3d3f7-8c42-4f20-83d9-46fdb520a61f_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_08c3d3f7-8c42-4f20-83d9-46fdb520a61f" xlink:to="loc_srt_NameOfMajorCustomerDomain_08c3d3f7-8c42-4f20-83d9-46fdb520a61f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_0614b64b-7dee-479b-96d5-e286a94ca074" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_08c3d3f7-8c42-4f20-83d9-46fdb520a61f" xlink:to="loc_srt_NameOfMajorCustomerDomain_0614b64b-7dee-479b-96d5-e286a94ca074" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_FiveCustomersMember_f0a86aa8-c1ab-4486-a2f3-264ac0b80a7b" xlink:href="tars-20240331.xsd#tars_FiveCustomersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_0614b64b-7dee-479b-96d5-e286a94ca074" xlink:to="loc_tars_FiveCustomersMember_f0a86aa8-c1ab-4486-a2f3-264ac0b80a7b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_2747c5b1-e711-4e98-9ee1-ca5443bf3196" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b2996b4c-eaea-4f32-a5b9-978a6f2157c1" xlink:to="loc_srt_ProductOrServiceAxis_2747c5b1-e711-4e98-9ee1-ca5443bf3196" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_2747c5b1-e711-4e98-9ee1-ca5443bf3196_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_2747c5b1-e711-4e98-9ee1-ca5443bf3196" xlink:to="loc_srt_ProductsAndServicesDomain_2747c5b1-e711-4e98-9ee1-ca5443bf3196_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_e53b83d9-fa72-4b63-aaea-ad5fb64d5087" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_2747c5b1-e711-4e98-9ee1-ca5443bf3196" xlink:to="loc_srt_ProductsAndServicesDomain_e53b83d9-fa72-4b63-aaea-ad5fb64d5087" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LicenseAndCollaborationOtherMember_86058c5f-3493-4f36-b19d-25a994cd7905" xlink:href="tars-20240331.xsd#tars_LicenseAndCollaborationOtherMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_e53b83d9-fa72-4b63-aaea-ad5fb64d5087" xlink:to="loc_tars_LicenseAndCollaborationOtherMember_86058c5f-3493-4f36-b19d-25a994cd7905" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_e3a69006-b419-4e89-b4b8-52f05bcef0fc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_e3a69006-b419-4e89-b4b8-52f05bcef0fc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_66995f9a-0567-4e37-bec9-282e06f18ec8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_66995f9a-0567-4e37-bec9-282e06f18ec8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_6fcd455f-06ee-4f72-b476-2d63e438aee3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_6fcd455f-06ee-4f72-b476-2d63e438aee3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardRequisiteServicePeriod1_2f06abbb-6a2d-453e-bb47-41e3ce391dc7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardRequisiteServicePeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardRequisiteServicePeriod1_2f06abbb-6a2d-453e-bb47-41e3ce391dc7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_0d745b96-6977-41a3-b6fa-4c79d2413a1a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_0d745b96-6977-41a3-b6fa-4c79d2413a1a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_bc617d06-fc1a-49fa-991e-6524c2232c24" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_Revenues_bc617d06-fc1a-49fa-991e-6524c2232c24" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_e2d7707d-fa5b-4991-83e6-2b77212d2313" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_e2d7707d-fa5b-4991-83e6-2b77212d2313" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_5ecdde2f-0531-450f-b156-dc80eef0b611" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_9562b999-c44d-4bfd-b900-a7e078e45795" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_5ecdde2f-0531-450f-b156-dc80eef0b611" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/SummaryofSignificantAccountingPoliciesandUseofEstimatesProductSalesfromEachofourLargestCustomersDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#SummaryofSignificantAccountingPoliciesandUseofEstimatesProductSalesfromEachofourLargestCustomersDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/SummaryofSignificantAccountingPoliciesandUseofEstimatesProductSalesfromEachofourLargestCustomersDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_0aeb7364-d954-417a-a47c-58c39cdb7ca1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_41fe9736-e6b4-43a1-a7f4-ead9e6cf7deb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_0aeb7364-d954-417a-a47c-58c39cdb7ca1" xlink:to="loc_us-gaap_ConcentrationRiskTable_41fe9736-e6b4-43a1-a7f4-ead9e6cf7deb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_00beea65-e63d-45a4-bff5-f960d47b3dd9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_41fe9736-e6b4-43a1-a7f4-ead9e6cf7deb" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_00beea65-e63d-45a4-bff5-f960d47b3dd9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_00beea65-e63d-45a4-bff5-f960d47b3dd9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_00beea65-e63d-45a4-bff5-f960d47b3dd9" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_00beea65-e63d-45a4-bff5-f960d47b3dd9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_333ce2d8-9a84-4020-a765-2c1074f955e7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_00beea65-e63d-45a4-bff5-f960d47b3dd9" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_333ce2d8-9a84-4020-a765-2c1074f955e7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesRevenueNetMember_c86041fb-87f8-4132-b0ce-21a4445fa8f4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SalesRevenueNetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_333ce2d8-9a84-4020-a765-2c1074f955e7" xlink:to="loc_us-gaap_SalesRevenueNetMember_c86041fb-87f8-4132-b0ce-21a4445fa8f4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_60c5bcb0-ac03-440e-9c91-8a53100c3b90" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_41fe9736-e6b4-43a1-a7f4-ead9e6cf7deb" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_60c5bcb0-ac03-440e-9c91-8a53100c3b90" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_60c5bcb0-ac03-440e-9c91-8a53100c3b90_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_60c5bcb0-ac03-440e-9c91-8a53100c3b90" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_60c5bcb0-ac03-440e-9c91-8a53100c3b90_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_4701f5dc-21e4-42df-bd25-0ec6943fa489" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_60c5bcb0-ac03-440e-9c91-8a53100c3b90" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_4701f5dc-21e4-42df-bd25-0ec6943fa489" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_f648c296-6460-4df5-991b-68e60f65c148" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CustomerConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_4701f5dc-21e4-42df-bd25-0ec6943fa489" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_f648c296-6460-4df5-991b-68e60f65c148" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_607592af-f48e-40bd-9f08-148e357c7ae0" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_41fe9736-e6b4-43a1-a7f4-ead9e6cf7deb" xlink:to="loc_srt_MajorCustomersAxis_607592af-f48e-40bd-9f08-148e357c7ae0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_607592af-f48e-40bd-9f08-148e357c7ae0_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_607592af-f48e-40bd-9f08-148e357c7ae0" xlink:to="loc_srt_NameOfMajorCustomerDomain_607592af-f48e-40bd-9f08-148e357c7ae0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_e8434d64-4ee5-42b5-93f1-fd51dff92eeb" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_607592af-f48e-40bd-9f08-148e357c7ae0" xlink:to="loc_srt_NameOfMajorCustomerDomain_e8434d64-4ee5-42b5-93f1-fd51dff92eeb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CustomerAMember_a7d8d214-6bfe-4ace-92a6-a1ac5a4633b0" xlink:href="tars-20240331.xsd#tars_CustomerAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_e8434d64-4ee5-42b5-93f1-fd51dff92eeb" xlink:to="loc_tars_CustomerAMember_a7d8d214-6bfe-4ace-92a6-a1ac5a4633b0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CustomerBMember_06ddebba-6bdc-4113-bc4c-27f6e65810c5" xlink:href="tars-20240331.xsd#tars_CustomerBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_e8434d64-4ee5-42b5-93f1-fd51dff92eeb" xlink:to="loc_tars_CustomerBMember_06ddebba-6bdc-4113-bc4c-27f6e65810c5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CustomerCMember_16aa4728-92a7-4361-add7-0991d52c4875" xlink:href="tars-20240331.xsd#tars_CustomerCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_e8434d64-4ee5-42b5-93f1-fd51dff92eeb" xlink:to="loc_tars_CustomerCMember_16aa4728-92a7-4361-add7-0991d52c4875" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CustomerDMember_d2b9d283-8e59-4a61-930f-9a7aa145158d" xlink:href="tars-20240331.xsd#tars_CustomerDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_e8434d64-4ee5-42b5-93f1-fd51dff92eeb" xlink:to="loc_tars_CustomerDMember_d2b9d283-8e59-4a61-930f-9a7aa145158d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CustomerEMember_5e97b9b5-fe62-4832-ba24-3b09a096f46b" xlink:href="tars-20240331.xsd#tars_CustomerEMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_e8434d64-4ee5-42b5-93f1-fd51dff92eeb" xlink:to="loc_tars_CustomerEMember_5e97b9b5-fe62-4832-ba24-3b09a096f46b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_97f58229-ac2d-4949-8c07-f964be050234" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_0aeb7364-d954-417a-a47c-58c39cdb7ca1" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_97f58229-ac2d-4949-8c07-f964be050234" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/FairValueMeasurementsFinancialInstrumentsMeasuredatFairValueDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#FairValueMeasurementsFinancialInstrumentsMeasuredatFairValueDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/FairValueMeasurementsFinancialInstrumentsMeasuredatFairValueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_7e294477-ca85-4f73-b685-0b484aa42c7a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByBalanceSheetGroupingTable_ee6cdbfc-83a7-4024-ad3e-26a43b5f720f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByBalanceSheetGroupingTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_7e294477-ca85-4f73-b685-0b484aa42c7a" xlink:to="loc_us-gaap_FairValueByBalanceSheetGroupingTable_ee6cdbfc-83a7-4024-ad3e-26a43b5f720f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_9981d55f-7d02-474c-9d37-421a0d0c37bd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_ee6cdbfc-83a7-4024-ad3e-26a43b5f720f" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_9981d55f-7d02-474c-9d37-421a0d0c37bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_9981d55f-7d02-474c-9d37-421a0d0c37bd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_9981d55f-7d02-474c-9d37-421a0d0c37bd" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_9981d55f-7d02-474c-9d37-421a0d0c37bd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_97fe4679-1eba-4551-9403-67b13ab92f40" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_9981d55f-7d02-474c-9d37-421a0d0c37bd" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_97fe4679-1eba-4551-9403-67b13ab92f40" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_27ed4f2f-3b70-49a0-862f-5efef4036eeb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_97fe4679-1eba-4551-9403-67b13ab92f40" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_27ed4f2f-3b70-49a0-862f-5efef4036eeb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_07cc3953-36fd-452a-8386-73c927aeeeff" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_97fe4679-1eba-4551-9403-67b13ab92f40" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_07cc3953-36fd-452a-8386-73c927aeeeff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_14672208-b5da-4e5f-a462-3916097a5d26" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_97fe4679-1eba-4551-9403-67b13ab92f40" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_14672208-b5da-4e5f-a462-3916097a5d26" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_4f381721-d000-479d-a440-2cb7229dce7d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_ee6cdbfc-83a7-4024-ad3e-26a43b5f720f" xlink:to="loc_us-gaap_FinancialInstrumentAxis_4f381721-d000-479d-a440-2cb7229dce7d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4f381721-d000-479d-a440-2cb7229dce7d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_4f381721-d000-479d-a440-2cb7229dce7d" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4f381721-d000-479d-a440-2cb7229dce7d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b223ed8d-f1d5-40a9-bef4-227142767a08" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_4f381721-d000-479d-a440-2cb7229dce7d" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b223ed8d-f1d5-40a9-bef4-227142767a08" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_8605e55a-82d0-40c8-8a9b-24d023e966b2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b223ed8d-f1d5-40a9-bef4-227142767a08" xlink:to="loc_us-gaap_MoneyMarketFundsMember_8605e55a-82d0-40c8-8a9b-24d023e966b2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_628e3caa-ac1f-406d-970e-9b857d39348e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b223ed8d-f1d5-40a9-bef4-227142767a08" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_628e3caa-ac1f-406d-970e-9b857d39348e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperMember_a8ac576b-051a-410e-8bf2-4700df7720e8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommercialPaperMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b223ed8d-f1d5-40a9-bef4-227142767a08" xlink:to="loc_us-gaap_CommercialPaperMember_a8ac576b-051a-410e-8bf2-4700df7720e8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_91f0ef73-a367-456f-9b2c-74e772c9bcc8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b223ed8d-f1d5-40a9-bef4-227142767a08" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_91f0ef73-a367-456f-9b2c-74e772c9bcc8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentDebtSecuritiesMember_3857205a-a947-4fac-8a2a-6e083532c00b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_USGovernmentDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b223ed8d-f1d5-40a9-bef4-227142767a08" xlink:to="loc_us-gaap_USGovernmentDebtSecuritiesMember_3857205a-a947-4fac-8a2a-6e083532c00b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_cb174fb4-24bf-4803-93b5-8ef97743c684" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_7e294477-ca85-4f73-b685-0b484aa42c7a" xlink:to="loc_us-gaap_AssetsAbstract_cb174fb4-24bf-4803-93b5-8ef97743c684" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_fd70c8cd-cab3-4be8-b434-763f92a6efb6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_cb174fb4-24bf-4803-93b5-8ef97743c684" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_fd70c8cd-cab3-4be8-b434-763f92a6efb6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_DebtSecuritiesAvailableForSaleAndCashAndCashEquivalents_3fa021da-822c-4555-bb3d-8468da4c6993" xlink:href="tars-20240331.xsd#tars_DebtSecuritiesAvailableForSaleAndCashAndCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_cb174fb4-24bf-4803-93b5-8ef97743c684" xlink:to="loc_tars_DebtSecuritiesAvailableForSaleAndCashAndCashEquivalents_3fa021da-822c-4555-bb3d-8468da4c6993" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent_246d5251-d6f7-4468-970f-f59c79ecc038" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_cb174fb4-24bf-4803-93b5-8ef97743c684" xlink:to="loc_us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent_246d5251-d6f7-4468-970f-f59c79ecc038" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_EquityWarrantsFairValueDisclosure_3cfc3cba-7d2e-4d88-af9c-c0a3ad2ed685" xlink:href="tars-20240331.xsd#tars_EquityWarrantsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_cb174fb4-24bf-4803-93b5-8ef97743c684" xlink:to="loc_tars_EquityWarrantsFairValueDisclosure_3cfc3cba-7d2e-4d88-af9c-c0a3ad2ed685" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_510ab7f0-d698-4b24-be86-43e326c43b74" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_cb174fb4-24bf-4803-93b5-8ef97743c684" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_510ab7f0-d698-4b24-be86-43e326c43b74" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/FairValueMeasurementsAdditionalInformationDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#FairValueMeasurementsAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/FairValueMeasurementsAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_dfa3cf45-9307-4fbd-831d-e6fdeeb13acd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_dfa3cf45-9307-4fbd-831d-e6fdeeb13acd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_b70e607d-3b36-4d0a-b122-17ddbfc1fa87" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_dfa3cf45-9307-4fbd-831d-e6fdeeb13acd" xlink:to="loc_srt_RangeAxis_b70e607d-3b36-4d0a-b122-17ddbfc1fa87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_b70e607d-3b36-4d0a-b122-17ddbfc1fa87_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_b70e607d-3b36-4d0a-b122-17ddbfc1fa87" xlink:to="loc_srt_RangeMember_b70e607d-3b36-4d0a-b122-17ddbfc1fa87_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_787dcb26-b19f-41c6-9a6b-1a79c4979918" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_b70e607d-3b36-4d0a-b122-17ddbfc1fa87" xlink:to="loc_srt_RangeMember_787dcb26-b19f-41c6-9a6b-1a79c4979918" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_1b3a20da-24e9-4138-ba43-723cfb1e882f" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_787dcb26-b19f-41c6-9a6b-1a79c4979918" xlink:to="loc_srt_MinimumMember_1b3a20da-24e9-4138-ba43-723cfb1e882f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_0d6a85ee-8c8c-447e-98e3-e9781d6fdbea" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_787dcb26-b19f-41c6-9a6b-1a79c4979918" xlink:to="loc_srt_MaximumMember_0d6a85ee-8c8c-447e-98e3-e9781d6fdbea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_321384c3-9725-47d9-924e-a6aff641a151" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_dfa3cf45-9307-4fbd-831d-e6fdeeb13acd" xlink:to="loc_us-gaap_FinancialInstrumentAxis_321384c3-9725-47d9-924e-a6aff641a151" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_321384c3-9725-47d9-924e-a6aff641a151_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_321384c3-9725-47d9-924e-a6aff641a151" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_321384c3-9725-47d9-924e-a6aff641a151_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_484d6079-7a23-4e95-a7d8-9781c640119e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_321384c3-9725-47d9-924e-a6aff641a151" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_484d6079-7a23-4e95-a7d8-9781c640119e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_AvailableForSaleDebtSecuritiesContractualMaturityLessThan12MonthsMember_b096007a-d798-4639-8a41-b938e9baf1b0" xlink:href="tars-20240331.xsd#tars_AvailableForSaleDebtSecuritiesContractualMaturityLessThan12MonthsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_484d6079-7a23-4e95-a7d8-9781c640119e" xlink:to="loc_tars_AvailableForSaleDebtSecuritiesContractualMaturityLessThan12MonthsMember_b096007a-d798-4639-8a41-b938e9baf1b0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_e32b5f98-33c2-4f79-8494-1ff1db0c379b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_dfa3cf45-9307-4fbd-831d-e6fdeeb13acd" xlink:to="loc_dei_LegalEntityAxis_e32b5f98-33c2-4f79-8494-1ff1db0c379b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_e32b5f98-33c2-4f79-8494-1ff1db0c379b_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_e32b5f98-33c2-4f79-8494-1ff1db0c379b" xlink:to="loc_dei_EntityDomain_e32b5f98-33c2-4f79-8494-1ff1db0c379b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_ce79fdce-61b6-4d29-8cb1-93707cb657ad" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_e32b5f98-33c2-4f79-8494-1ff1db0c379b" xlink:to="loc_dei_EntityDomain_ce79fdce-61b6-4d29-8cb1-93707cb657ad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LianBioMember_a585032a-9d48-4632-82b8-e2364ae88d0a" xlink:href="tars-20240331.xsd#tars_LianBioMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_ce79fdce-61b6-4d29-8cb1-93707cb657ad" xlink:to="loc_tars_LianBioMember_a585032a-9d48-4632-82b8-e2364ae88d0a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_5949f539-bb4b-4e6d-8661-e491136d3e34" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_dfa3cf45-9307-4fbd-831d-e6fdeeb13acd" xlink:to="loc_srt_CounterpartyNameAxis_5949f539-bb4b-4e6d-8661-e491136d3e34" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_5949f539-bb4b-4e6d-8661-e491136d3e34_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_5949f539-bb4b-4e6d-8661-e491136d3e34" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_5949f539-bb4b-4e6d-8661-e491136d3e34_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c9840037-d29b-49ae-aa10-340ec0beddac" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_5949f539-bb4b-4e6d-8661-e491136d3e34" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c9840037-d29b-49ae-aa10-340ec0beddac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LianBioMember_51bc5e92-d02b-401a-b538-2fb25bdb213d" xlink:href="tars-20240331.xsd#tars_LianBioMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c9840037-d29b-49ae-aa10-340ec0beddac" xlink:to="loc_tars_LianBioMember_51bc5e92-d02b-401a-b538-2fb25bdb213d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_f84f89e3-7a3f-44de-b7da-f89f40c1bcb2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TypeOfArrangementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_dfa3cf45-9307-4fbd-831d-e6fdeeb13acd" xlink:to="loc_us-gaap_TypeOfArrangementAxis_f84f89e3-7a3f-44de-b7da-f89f40c1bcb2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_f84f89e3-7a3f-44de-b7da-f89f40c1bcb2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_f84f89e3-7a3f-44de-b7da-f89f40c1bcb2" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_f84f89e3-7a3f-44de-b7da-f89f40c1bcb2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_5a308575-b22b-41cc-8b13-ca29205bd453" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_f84f89e3-7a3f-44de-b7da-f89f40c1bcb2" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_5a308575-b22b-41cc-8b13-ca29205bd453" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember_03678a36-6e35-47f1-bec5-2a953ebbd8ff" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_5a308575-b22b-41cc-8b13-ca29205bd453" xlink:to="loc_us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember_03678a36-6e35-47f1-bec5-2a953ebbd8ff" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_WarrantAgreementNumberOfTranches_96d48e26-9d41-45b4-945f-5d4b7543d63f" xlink:href="tars-20240331.xsd#tars_WarrantAgreementNumberOfTranches"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_tars_WarrantAgreementNumberOfTranches_96d48e26-9d41-45b4-945f-5d4b7543d63f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_NumberOfVestedWarrants_14bf52d0-dab0-4dc3-a790-590ae32506b1" xlink:href="tars-20240331.xsd#tars_NumberOfVestedWarrants"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_tars_NumberOfVestedWarrants_14bf52d0-dab0-4dc3-a790-590ae32506b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_EquitySecuritiesFVNINumberOfShares_26e11b07-d936-4aff-9c80-75f2fab54a6c" xlink:href="tars-20240331.xsd#tars_EquitySecuritiesFVNINumberOfShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_tars_EquitySecuritiesFVNINumberOfShares_26e11b07-d936-4aff-9c80-75f2fab54a6c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsCommonStockCash_ec292c47-1c36-47d2-8299-c1e30e4b06c2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DividendsCommonStockCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_us-gaap_DividendsCommonStockCash_ec292c47-1c36-47d2-8299-c1e30e4b06c2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareCashPaid_95cfa70c-5fff-493c-b3f3-5a448a0272ca" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockDividendsPerShareCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_us-gaap_CommonStockDividendsPerShareCashPaid_95cfa70c-5fff-493c-b3f3-5a448a0272ca" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ProceedsFromCancellationOfWarrants_fdef852b-1674-4c3a-a3a9-59cfacb4890c" xlink:href="tars-20240331.xsd#tars_ProceedsFromCancellationOfWarrants"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_tars_ProceedsFromCancellationOfWarrants_fdef852b-1674-4c3a-a3a9-59cfacb4890c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_DebtSecuritiesAvailableForSaleContractualMaturitiesGreaterThanTwelveMonthsNumberOfSecurities_e1a04c3f-af14-41b6-91dc-36f64cdc3275" xlink:href="tars-20240331.xsd#tars_DebtSecuritiesAvailableForSaleContractualMaturitiesGreaterThanTwelveMonthsNumberOfSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_tars_DebtSecuritiesAvailableForSaleContractualMaturitiesGreaterThanTwelveMonthsNumberOfSecurities_e1a04c3f-af14-41b6-91dc-36f64cdc3275" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTerm_b859c249-de0c-4894-a742-90abc4b0e176" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtSecuritiesAvailableForSaleTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTerm_b859c249-de0c-4894-a742-90abc4b0e176" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_049f290e-653c-48f3-a011-72eedd6c5e2e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_049f290e-653c-48f3-a011-72eedd6c5e2e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_00e75658-1625-4d4e-9aa8-f434d489e59c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_00e75658-1625-4d4e-9aa8-f434d489e59c" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsNumberOfPositions_61832307-1500-40e2-8045-4ee6350d3fa5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsNumberOfPositions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_6dc8b8ac-defe-4580-9080-9389014fc5dd" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsNumberOfPositions_61832307-1500-40e2-8045-4ee6350d3fa5" xlink:type="arc" order="10"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/FairValueMeasurementsEstimatedValueofCashCashEquivalentsMarketableSecuritiesandEquitySecuritiesDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#FairValueMeasurementsEstimatedValueofCashCashEquivalentsMarketableSecuritiesandEquitySecuritiesDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/FairValueMeasurementsEstimatedValueofCashCashEquivalentsMarketableSecuritiesandEquitySecuritiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_add8f29b-c00b-44bc-8826-361124c1213d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6854199b-7133-4066-b0e9-2e3dd7aa86ff" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_add8f29b-c00b-44bc-8826-361124c1213d" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6854199b-7133-4066-b0e9-2e3dd7aa86ff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_c4349842-bbc2-4d57-8ec7-d5c624b1d6ec" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6854199b-7133-4066-b0e9-2e3dd7aa86ff" xlink:to="loc_us-gaap_FinancialInstrumentAxis_c4349842-bbc2-4d57-8ec7-d5c624b1d6ec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c4349842-bbc2-4d57-8ec7-d5c624b1d6ec_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_c4349842-bbc2-4d57-8ec7-d5c624b1d6ec" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c4349842-bbc2-4d57-8ec7-d5c624b1d6ec_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd7fab47-8a74-4b1f-8d4d-17ceca3486a9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_c4349842-bbc2-4d57-8ec7-d5c624b1d6ec" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd7fab47-8a74-4b1f-8d4d-17ceca3486a9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_12a43b1e-4b6a-48a1-9c25-8401879a760a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd7fab47-8a74-4b1f-8d4d-17ceca3486a9" xlink:to="loc_us-gaap_MoneyMarketFundsMember_12a43b1e-4b6a-48a1-9c25-8401879a760a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_c38d652c-9508-49ad-9bbd-bc65ebcd0046" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd7fab47-8a74-4b1f-8d4d-17ceca3486a9" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_c38d652c-9508-49ad-9bbd-bc65ebcd0046" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperMember_4143408c-a7da-4ecf-b867-cfbeb32a9ee4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommercialPaperMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd7fab47-8a74-4b1f-8d4d-17ceca3486a9" xlink:to="loc_us-gaap_CommercialPaperMember_4143408c-a7da-4ecf-b867-cfbeb32a9ee4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_8e983d64-0a8c-4dd6-96fc-0d6fd98075e2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd7fab47-8a74-4b1f-8d4d-17ceca3486a9" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_8e983d64-0a8c-4dd6-96fc-0d6fd98075e2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentDebtSecuritiesMember_f4b50e77-bfe8-432c-9ea6-1a314f6bf311" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_USGovernmentDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd7fab47-8a74-4b1f-8d4d-17ceca3486a9" xlink:to="loc_us-gaap_USGovernmentDebtSecuritiesMember_f4b50e77-bfe8-432c-9ea6-1a314f6bf311" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CommonStockInLianBioMember_56a85913-a061-4569-b6b0-50e800461219" xlink:href="tars-20240331.xsd#tars_CommonStockInLianBioMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd7fab47-8a74-4b1f-8d4d-17ceca3486a9" xlink:to="loc_tars_CommonStockInLianBioMember_56a85913-a061-4569-b6b0-50e800461219" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationAxis_2a195b3b-eadd-4c9d-8e6c-5330476e7cb6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BalanceSheetLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_6854199b-7133-4066-b0e9-2e3dd7aa86ff" xlink:to="loc_us-gaap_BalanceSheetLocationAxis_2a195b3b-eadd-4c9d-8e6c-5330476e7cb6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_2a195b3b-eadd-4c9d-8e6c-5330476e7cb6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_2a195b3b-eadd-4c9d-8e6c-5330476e7cb6" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_2a195b3b-eadd-4c9d-8e6c-5330476e7cb6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_eabf2a72-2a2f-4ede-b9f1-ce1e3bb09e03" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_2a195b3b-eadd-4c9d-8e6c-5330476e7cb6" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_eabf2a72-2a2f-4ede-b9f1-ce1e3bb09e03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashEquivalentsMember_2840ad71-a19d-490d-9cde-ca9d456bbaed" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_eabf2a72-2a2f-4ede-b9f1-ce1e3bb09e03" xlink:to="loc_us-gaap_CashEquivalentsMember_2840ad71-a19d-490d-9cde-ca9d456bbaed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAbstract_5099ed15-c4ec-4a9d-80d8-4b5d789bafef" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_add8f29b-c00b-44bc-8826-361124c1213d" xlink:to="loc_us-gaap_CashAndCashEquivalentsAbstract_5099ed15-c4ec-4a9d-80d8-4b5d789bafef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_1f596201-feeb-4252-a215-875518159309" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsAbstract_5099ed15-c4ec-4a9d-80d8-4b5d789bafef" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_1f596201-feeb-4252-a215-875518159309" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_b9c8a2da-22d6-4aa0-8496-c99829367f7f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsAbstract_5099ed15-c4ec-4a9d-80d8-4b5d789bafef" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_b9c8a2da-22d6-4aa0-8496-c99829367f7f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesAbstract_01cb99e2-4046-4494-b9fc-abdc7184d834" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_add8f29b-c00b-44bc-8826-361124c1213d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesAbstract_01cb99e2-4046-4494-b9fc-abdc7184d834" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_461784ea-06d6-4234-b4c9-df02d84b057c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_01cb99e2-4046-4494-b9fc-abdc7184d834" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_461784ea-06d6-4234-b4c9-df02d84b057c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_c1f6a954-cce3-4e2c-ad22-987f27249fa5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_01cb99e2-4046-4494-b9fc-abdc7184d834" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_c1f6a954-cce3-4e2c-ad22-987f27249fa5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_4fecda66-0a9e-4a4d-94f2-b40aab9f317c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_01cb99e2-4046-4494-b9fc-abdc7184d834" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_4fecda66-0a9e-4a4d-94f2-b40aab9f317c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_6def5b83-57a0-43da-ac1d-70226119b5fc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_01cb99e2-4046-4494-b9fc-abdc7184d834" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_6def5b83-57a0-43da-ac1d-70226119b5fc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFVNIAbstract_d3b595a0-070f-4de3-90ec-7948753d4190" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesFVNIAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_add8f29b-c00b-44bc-8826-361124c1213d" xlink:to="loc_us-gaap_EquitySecuritiesFVNIAbstract_d3b595a0-070f-4de3-90ec-7948753d4190" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiCost_8cc4de75-21df-4a58-a6ea-dfc0b924b074" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesFvNiCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquitySecuritiesFVNIAbstract_d3b595a0-070f-4de3-90ec-7948753d4190" xlink:to="loc_us-gaap_EquitySecuritiesFvNiCost_8cc4de75-21df-4a58-a6ea-dfc0b924b074" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiUnrealizedGain_1c5ab593-3a63-4e5d-bd39-35394aec6b34" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesFvNiUnrealizedGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquitySecuritiesFVNIAbstract_d3b595a0-070f-4de3-90ec-7948753d4190" xlink:to="loc_us-gaap_EquitySecuritiesFvNiUnrealizedGain_1c5ab593-3a63-4e5d-bd39-35394aec6b34" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiUnrealizedLoss_aa7d2a49-ec71-4a40-8130-ab4abca7b7ed" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesFvNiUnrealizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquitySecuritiesFVNIAbstract_d3b595a0-070f-4de3-90ec-7948753d4190" xlink:to="loc_us-gaap_EquitySecuritiesFvNiUnrealizedLoss_aa7d2a49-ec71-4a40-8130-ab4abca7b7ed" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent_952b9e61-3b39-4324-b36a-f4bf7a5e6ab2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquitySecuritiesFVNIAbstract_d3b595a0-070f-4de3-90ec-7948753d4190" xlink:to="loc_us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent_952b9e61-3b39-4324-b36a-f4bf7a5e6ab2" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/BalanceSheetAccountDetailPropertyandEquipmentNetofAccumulatedDepreciationDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#BalanceSheetAccountDetailPropertyandEquipmentNetofAccumulatedDepreciationDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/BalanceSheetAccountDetailPropertyandEquipmentNetofAccumulatedDepreciationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_ac9ed1eb-4472-46e1-a47d-20d96b600e0a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_6037db1f-a869-4c4d-a45b-6c4e4c5a478d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_ac9ed1eb-4472-46e1-a47d-20d96b600e0a" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_6037db1f-a869-4c4d-a45b-6c4e4c5a478d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_8f87f5a4-22bf-41b3-b1ed-979db1ab770a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_6037db1f-a869-4c4d-a45b-6c4e4c5a478d" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_8f87f5a4-22bf-41b3-b1ed-979db1ab770a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_8f87f5a4-22bf-41b3-b1ed-979db1ab770a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_8f87f5a4-22bf-41b3-b1ed-979db1ab770a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_8f87f5a4-22bf-41b3-b1ed-979db1ab770a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_63483405-2be4-4fc3-a59c-201f050bb8cd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_8f87f5a4-22bf-41b3-b1ed-979db1ab770a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_63483405-2be4-4fc3-a59c-201f050bb8cd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_555cd4e8-4124-4abf-babe-960e275b4022" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_63483405-2be4-4fc3-a59c-201f050bb8cd" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_555cd4e8-4124-4abf-babe-960e275b4022" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OfficeEquipmentMember_e1d67226-96d3-46ac-beff-ba3dd1452e63" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OfficeEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_63483405-2be4-4fc3-a59c-201f050bb8cd" xlink:to="loc_us-gaap_OfficeEquipmentMember_e1d67226-96d3-46ac-beff-ba3dd1452e63" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherMachineryAndEquipmentMember_66393c08-8419-4e14-af26-1376a22e2481" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherMachineryAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_63483405-2be4-4fc3-a59c-201f050bb8cd" xlink:to="loc_us-gaap_OtherMachineryAndEquipmentMember_66393c08-8419-4e14-af26-1376a22e2481" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_fcfd3a4a-87ea-400a-be45-210e26ba9bc1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_63483405-2be4-4fc3-a59c-201f050bb8cd" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_fcfd3a4a-87ea-400a-be45-210e26ba9bc1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_464ed6f3-69dc-4d2f-bd21-9f65616fe400" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_ac9ed1eb-4472-46e1-a47d-20d96b600e0a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_464ed6f3-69dc-4d2f-bd21-9f65616fe400" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1f6b935e-83ce-4a43-b758-b0833f7aea7f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_ac9ed1eb-4472-46e1-a47d-20d96b600e0a" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1f6b935e-83ce-4a43-b758-b0833f7aea7f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_865485db-d9e0-41c5-a950-a1535adc236c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_ac9ed1eb-4472-46e1-a47d-20d96b600e0a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_865485db-d9e0-41c5-a950-a1535adc236c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Depreciation_47fbef7d-e5f9-4b1b-b7b3-92efe1b5878a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Depreciation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_ac9ed1eb-4472-46e1-a47d-20d96b600e0a" xlink:to="loc_us-gaap_Depreciation_47fbef7d-e5f9-4b1b-b7b3-92efe1b5878a" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/StockBasedCompensationAdditionalInformationDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#StockBasedCompensationAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/StockBasedCompensationAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_160a8f22-d625-40a4-8f58-621ff4d923ec" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_160a8f22-d625-40a4-8f58-621ff4d923ec" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_2ee87988-699f-4abc-ac5c-1dc7508d3760" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_160a8f22-d625-40a4-8f58-621ff4d923ec" xlink:to="loc_us-gaap_AwardTypeAxis_2ee87988-699f-4abc-ac5c-1dc7508d3760" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2ee87988-699f-4abc-ac5c-1dc7508d3760_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_2ee87988-699f-4abc-ac5c-1dc7508d3760" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2ee87988-699f-4abc-ac5c-1dc7508d3760_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_81d0ded0-1e2d-4960-a8ac-d7daa0ce3ba9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_2ee87988-699f-4abc-ac5c-1dc7508d3760" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_81d0ded0-1e2d-4960-a8ac-d7daa0ce3ba9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_49c37a13-fb69-49bc-adaa-7eb7fde59772" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_81d0ded0-1e2d-4960-a8ac-d7daa0ce3ba9" xlink:to="loc_us-gaap_EmployeeStockOptionMember_49c37a13-fb69-49bc-adaa-7eb7fde59772" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_c2648932-5835-446b-898e-d59aacb3d63f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_81d0ded0-1e2d-4960-a8ac-d7daa0ce3ba9" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_c2648932-5835-446b-898e-d59aacb3d63f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_cff73be5-85fc-46d0-9790-9d4f9b732954" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_81d0ded0-1e2d-4960-a8ac-d7daa0ce3ba9" xlink:to="loc_us-gaap_EmployeeStockMember_cff73be5-85fc-46d0-9790-9d4f9b732954" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_af929f97-c085-43a4-aad8-7970de8913ca" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_af929f97-c085-43a4-aad8-7970de8913ca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ClassOfWarrantOrRightReservedForFutureIssuance_d846f242-9815-40ef-bab1-c515411425e8" xlink:href="tars-20240331.xsd#tars_ClassOfWarrantOrRightReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_tars_ClassOfWarrantOrRightReservedForFutureIssuance_d846f242-9815-40ef-bab1-c515411425e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_66e8c738-faa1-4687-b674-f859309f36d5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_us-gaap_CommonStockSharesIssued_66e8c738-faa1-4687-b674-f859309f36d5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CommonStockNumberOfVotes_2361a0eb-679d-4a39-a58d-6b78e80a31ec" xlink:href="tars-20240331.xsd#tars_CommonStockNumberOfVotes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_tars_CommonStockNumberOfVotes_2361a0eb-679d-4a39-a58d-6b78e80a31ec" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_2187b5c7-a351-4358-aaaf-9846b08a1a7c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_2187b5c7-a351-4358-aaaf-9846b08a1a7c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_6caef0b9-4659-4e07-8831-6ab2e69124d0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_6caef0b9-4659-4e07-8831-6ab2e69124d0" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_ab871711-ac1e-47f1-914c-f7e415855888" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_ab871711-ac1e-47f1-914c-f7e415855888" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_c8b9258d-641d-4952-820f-edf69bf355ff" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_20135668-d76a-4210-859f-e760f993a345" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_c8b9258d-641d-4952-820f-edf69bf355ff" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/StockBasedCompensationExpenseDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#StockBasedCompensationExpenseDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/StockBasedCompensationExpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_4cb8af68-d03e-4008-a846-5dd31df5a99a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_701c3eca-92a9-456f-9bd1-386c936b9405" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_4cb8af68-d03e-4008-a846-5dd31df5a99a" xlink:to="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_701c3eca-92a9-456f-9bd1-386c936b9405" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_4eb561b5-3879-4eb7-ba1a-b331b59388cc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_701c3eca-92a9-456f-9bd1-386c936b9405" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_4eb561b5-3879-4eb7-ba1a-b331b59388cc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_4eb561b5-3879-4eb7-ba1a-b331b59388cc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_4eb561b5-3879-4eb7-ba1a-b331b59388cc" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_4eb561b5-3879-4eb7-ba1a-b331b59388cc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_56bf58c7-47e7-4154-a498-6762257c8338" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_4eb561b5-3879-4eb7-ba1a-b331b59388cc" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_56bf58c7-47e7-4154-a498-6762257c8338" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_c24db05d-4301-485b-a0f1-2a4f911c42bb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_56bf58c7-47e7-4154-a498-6762257c8338" xlink:to="loc_us-gaap_CostOfSalesMember_c24db05d-4301-485b-a0f1-2a4f911c42bb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_0951a89e-41e0-49f7-8a6b-0e7bb3d0b83d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_56bf58c7-47e7-4154-a498-6762257c8338" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_0951a89e-41e0-49f7-8a6b-0e7bb3d0b83d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpensesMember_7fe3e25d-a5c7-46a3-86bb-28a09890cb05" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SellingGeneralAndAdministrativeExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_56bf58c7-47e7-4154-a498-6762257c8338" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpensesMember_7fe3e25d-a5c7-46a3-86bb-28a09890cb05" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_72418f2f-a27b-40c4-a22e-32a40d2ae285" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_4cb8af68-d03e-4008-a846-5dd31df5a99a" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_72418f2f-a27b-40c4-a22e-32a40d2ae285" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/StockBasedCompensationRestrictedStockUnitActivityDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#StockBasedCompensationRestrictedStockUnitActivityDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/StockBasedCompensationRestrictedStockUnitActivityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f17f29ae-b3ef-421d-8550-33ef65a4f41b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_2bbbfe46-c915-480b-b19e-f6f1b1162d45" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f17f29ae-b3ef-421d-8550-33ef65a4f41b" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_2bbbfe46-c915-480b-b19e-f6f1b1162d45" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_778aa2a1-39e8-499b-b885-03572d087b25" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_2bbbfe46-c915-480b-b19e-f6f1b1162d45" xlink:to="loc_us-gaap_AwardTypeAxis_778aa2a1-39e8-499b-b885-03572d087b25" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_778aa2a1-39e8-499b-b885-03572d087b25_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_778aa2a1-39e8-499b-b885-03572d087b25" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_778aa2a1-39e8-499b-b885-03572d087b25_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c027c488-18a8-45ff-80f9-04e77ea25b8d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_778aa2a1-39e8-499b-b885-03572d087b25" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c027c488-18a8-45ff-80f9-04e77ea25b8d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_b1c4c869-cb9b-4416-93e6-413bcb9bdfbc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c027c488-18a8-45ff-80f9-04e77ea25b8d" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_b1c4c869-cb9b-4416-93e6-413bcb9bdfbc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_79d4a62a-3580-49f1-90b7-3c898bde77f5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f17f29ae-b3ef-421d-8550-33ef65a4f41b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_79d4a62a-3580-49f1-90b7-3c898bde77f5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_59549e31-d1bf-423f-9036-46ebc020d917" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_79d4a62a-3580-49f1-90b7-3c898bde77f5" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_59549e31-d1bf-423f-9036-46ebc020d917" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_d0b801bc-9dcc-49d2-8c25-91d10203be2b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_79d4a62a-3580-49f1-90b7-3c898bde77f5" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_d0b801bc-9dcc-49d2-8c25-91d10203be2b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_53234cb7-56b0-4644-89c9-54e968ad2dc3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_79d4a62a-3580-49f1-90b7-3c898bde77f5" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_53234cb7-56b0-4644-89c9-54e968ad2dc3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_07a137d4-beb7-41ae-b836-29d7e39bffee" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_79d4a62a-3580-49f1-90b7-3c898bde77f5" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_07a137d4-beb7-41ae-b836-29d7e39bffee" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_467f5413-7a0f-4919-b664-a718e0db7506" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_abb43442-9e36-4d4f-8ea4-aab1614d17ac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_f17f29ae-b3ef-421d-8550-33ef65a4f41b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_abb43442-9e36-4d4f-8ea4-aab1614d17ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_376fc26e-3888-49b0-a7be-af90bb6e908b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_abb43442-9e36-4d4f-8ea4-aab1614d17ac" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_376fc26e-3888-49b0-a7be-af90bb6e908b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_77e9c731-adae-4bb2-8492-ad513a492530" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_abb43442-9e36-4d4f-8ea4-aab1614d17ac" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_77e9c731-adae-4bb2-8492-ad513a492530" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_4dc73054-ebcb-47fb-b5ab-0dd955c45c04" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_abb43442-9e36-4d4f-8ea4-aab1614d17ac" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_4dc73054-ebcb-47fb-b5ab-0dd955c45c04" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_d287d7e0-7462-4f72-a328-eedb34dcb9bb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_abb43442-9e36-4d4f-8ea4-aab1614d17ac" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_d287d7e0-7462-4f72-a328-eedb34dcb9bb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_275a2826-716f-4b92-ab44-5e810a574c63" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/NetLossPerShareAntidilutiveSecuritiesDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#NetLossPerShareAntidilutiveSecuritiesDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/NetLossPerShareAntidilutiveSecuritiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_48640ada-e106-4ad8-b207-1e24f4694628" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_a93082f5-dc29-4ecd-875d-65c2f9539d06" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_48640ada-e106-4ad8-b207-1e24f4694628" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_a93082f5-dc29-4ecd-875d-65c2f9539d06" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_b2ba1a6a-cc30-4ba3-8b99-d91146484458" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_a93082f5-dc29-4ecd-875d-65c2f9539d06" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_b2ba1a6a-cc30-4ba3-8b99-d91146484458" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_b2ba1a6a-cc30-4ba3-8b99-d91146484458_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_b2ba1a6a-cc30-4ba3-8b99-d91146484458" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_b2ba1a6a-cc30-4ba3-8b99-d91146484458_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_c8c514a8-028f-4be6-9e36-8ac80b377dc5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_b2ba1a6a-cc30-4ba3-8b99-d91146484458" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_c8c514a8-028f-4be6-9e36-8ac80b377dc5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_357a6c7a-846e-4067-b5fb-2c888d550f2e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_c8c514a8-028f-4be6-9e36-8ac80b377dc5" xlink:to="loc_us-gaap_EmployeeStockOptionMember_357a6c7a-846e-4067-b5fb-2c888d550f2e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ShareBasedPaymentArrangementEquityInstrumentsOtherThanOptionsRestrictedStockUnitsUnvestedMember_8ace0588-3020-477a-94e6-db465e0a6b0f" xlink:href="tars-20240331.xsd#tars_ShareBasedPaymentArrangementEquityInstrumentsOtherThanOptionsRestrictedStockUnitsUnvestedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_c8c514a8-028f-4be6-9e36-8ac80b377dc5" xlink:to="loc_tars_ShareBasedPaymentArrangementEquityInstrumentsOtherThanOptionsRestrictedStockUnitsUnvestedMember_8ace0588-3020-477a-94e6-db465e0a6b0f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_7740a183-dc82-4532-8d57-94b743fdcbbd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_48640ada-e106-4ad8-b207-1e24f4694628" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_7740a183-dc82-4532-8d57-94b743fdcbbd" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/CommitmentContingenciesInLicenseAgreementforLotilanerDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#CommitmentContingenciesInLicenseAgreementforLotilanerDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/CommitmentContingenciesInLicenseAgreementforLotilanerDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_86f2f26c-27ca-4de3-acae-7931caa98a23" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_86f2f26c-27ca-4de3-acae-7931caa98a23" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_474bd3df-e77d-4ab4-903b-aff89c524c47" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TypeOfArrangementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_86f2f26c-27ca-4de3-acae-7931caa98a23" xlink:to="loc_us-gaap_TypeOfArrangementAxis_474bd3df-e77d-4ab4-903b-aff89c524c47" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_474bd3df-e77d-4ab4-903b-aff89c524c47_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_474bd3df-e77d-4ab4-903b-aff89c524c47" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_474bd3df-e77d-4ab4-903b-aff89c524c47_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_7af5cda2-1345-4c4e-9c05-c409234f2501" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_474bd3df-e77d-4ab4-903b-aff89c524c47" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_7af5cda2-1345-4c4e-9c05-c409234f2501" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember_972213cf-ca1c-49aa-8b3a-a88180a7effb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_7af5cda2-1345-4c4e-9c05-c409234f2501" xlink:to="loc_us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember_972213cf-ca1c-49aa-8b3a-a88180a7effb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_9f10576e-8486-4c17-8733-c3e93e3e823c" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_86f2f26c-27ca-4de3-acae-7931caa98a23" xlink:to="loc_srt_ProductOrServiceAxis_9f10576e-8486-4c17-8733-c3e93e3e823c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_9f10576e-8486-4c17-8733-c3e93e3e823c_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_9f10576e-8486-4c17-8733-c3e93e3e823c" xlink:to="loc_srt_ProductsAndServicesDomain_9f10576e-8486-4c17-8733-c3e93e3e823c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_387ba7e7-198b-4db0-bb73-6bc978cfe3a7" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_9f10576e-8486-4c17-8733-c3e93e3e823c" xlink:to="loc_srt_ProductsAndServicesDomain_387ba7e7-198b-4db0-bb73-6bc978cfe3a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ClinicalMilestonesMember_1bcc9381-f9d6-41a8-96b6-5384cddb112b" xlink:href="tars-20240331.xsd#tars_ClinicalMilestonesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_387ba7e7-198b-4db0-bb73-6bc978cfe3a7" xlink:to="loc_tars_ClinicalMilestonesMember_1bcc9381-f9d6-41a8-96b6-5384cddb112b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CommercialAndSalesMilestonesMember_be572b64-1dfa-4174-a7ae-0d7a14e9a939" xlink:href="tars-20240331.xsd#tars_CommercialAndSalesMilestonesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_387ba7e7-198b-4db0-bb73-6bc978cfe3a7" xlink:to="loc_tars_CommercialAndSalesMilestonesMember_be572b64-1dfa-4174-a7ae-0d7a14e9a939" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_26a113c9-0fd9-43ad-9745-cd5f3427e61c" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_86f2f26c-27ca-4de3-acae-7931caa98a23" xlink:to="loc_srt_CounterpartyNameAxis_26a113c9-0fd9-43ad-9745-cd5f3427e61c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_26a113c9-0fd9-43ad-9745-cd5f3427e61c_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_26a113c9-0fd9-43ad-9745-cd5f3427e61c" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_26a113c9-0fd9-43ad-9745-cd5f3427e61c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_0b6ded23-b22a-45bc-b802-97faab8c1f89" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_26a113c9-0fd9-43ad-9745-cd5f3427e61c" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_0b6ded23-b22a-45bc-b802-97faab8c1f89" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ElancoMember_31820307-570a-4eaa-951a-f3b163fa5cf8" xlink:href="tars-20240331.xsd#tars_ElancoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_0b6ded23-b22a-45bc-b802-97faab8c1f89" xlink:to="loc_tars_ElancoMember_31820307-570a-4eaa-951a-f3b163fa5cf8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis_c82f1ab8-c952-4082-8b64-060f6cd513f4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_86f2f26c-27ca-4de3-acae-7931caa98a23" xlink:to="loc_us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis_c82f1ab8-c952-4082-8b64-060f6cd513f4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_c82f1ab8-c952-4082-8b64-060f6cd513f4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongLivedAssetsHeldForSaleNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis_c82f1ab8-c952-4082-8b64-060f6cd513f4" xlink:to="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_c82f1ab8-c952-4082-8b64-060f6cd513f4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_96f03869-8afe-49f7-9c61-4fc37be8cec1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongLivedAssetsHeldForSaleNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongLivedAssetsHeldForSaleByAssetTypeAxis_c82f1ab8-c952-4082-8b64-060f6cd513f4" xlink:to="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_96f03869-8afe-49f7-9c61-4fc37be8cec1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_IntangibleAssetsMilestonePaymentMember_fdeaf0ea-ea58-4fce-8e99-b768d76b82d6" xlink:href="tars-20240331.xsd#tars_IntangibleAssetsMilestonePaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongLivedAssetsHeldForSaleNameDomain_96f03869-8afe-49f7-9c61-4fc37be8cec1" xlink:to="loc_tars_IntangibleAssetsMilestonePaymentMember_fdeaf0ea-ea58-4fce-8e99-b768d76b82d6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CollaborativeArrangementRightsAndObligationsContractualMilestonePayment_5d2c38b5-8080-4f5e-a2fa-ca249fcf458b" xlink:href="tars-20240331.xsd#tars_CollaborativeArrangementRightsAndObligationsContractualMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_tars_CollaborativeArrangementRightsAndObligationsContractualMilestonePayment_5d2c38b5-8080-4f5e-a2fa-ca249fcf458b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CollaborativeArrangementRightsAndObligationsUpfrontPayment_3a52a699-33eb-4ba7-9c70-ff1e6cf5e0dd" xlink:href="tars-20240331.xsd#tars_CollaborativeArrangementRightsAndObligationsUpfrontPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_tars_CollaborativeArrangementRightsAndObligationsUpfrontPayment_3a52a699-33eb-4ba7-9c70-ff1e6cf5e0dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_cf30ade8-e000-4a7c-a962-2f71c92213ce" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_cf30ade8-e000-4a7c-a962-2f71c92213ce" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CollaborativeArrangementFutureCashPayments_93c27623-b1b8-4a8b-a65f-88583e798b8b" xlink:href="tars-20240331.xsd#tars_CollaborativeArrangementFutureCashPayments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_tars_CollaborativeArrangementFutureCashPayments_93c27623-b1b8-4a8b-a65f-88583e798b8b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CollaborativeArrangementMaximumMilestonePayment_826122d0-9ce4-4e3b-8081-a27541e1cfbd" xlink:href="tars-20240331.xsd#tars_CollaborativeArrangementMaximumMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_tars_CollaborativeArrangementMaximumMilestonePayment_826122d0-9ce4-4e3b-8081-a27541e1cfbd" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RoyaltyExpense_2e5b48bf-fe20-4695-8704-b2fbf7d9c2ad" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RoyaltyExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_us-gaap_RoyaltyExpense_2e5b48bf-fe20-4695-8704-b2fbf7d9c2ad" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_e1597376-30f5-48e9-bead-d19704571934" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesIssuedForServices"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_e1597376-30f5-48e9-bead-d19704571934" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueIssuedForServices_26cb311a-66f5-4062-9efc-ae10b1737296" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueIssuedForServices"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueIssuedForServices_26cb311a-66f5-4062-9efc-ae10b1737296" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharePrice_493dcea4-87ab-468a-990f-8d783bc9266e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SharePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_2fc2c757-d366-48e5-b1f7-4af20f4804aa" xlink:to="loc_us-gaap_SharePrice_493dcea4-87ab-468a-990f-8d783bc9266e" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/OutLicenseAgreementsDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#OutLicenseAgreementsDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/OutLicenseAgreementsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_b1c779c7-4f1c-4463-8582-d911d653adbc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_b1c779c7-4f1c-4463-8582-d911d653adbc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_b4b466c9-20d4-481f-af75-8691b26a588b" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_b1c779c7-4f1c-4463-8582-d911d653adbc" xlink:to="loc_srt_CounterpartyNameAxis_b4b466c9-20d4-481f-af75-8691b26a588b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_b4b466c9-20d4-481f-af75-8691b26a588b_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_b4b466c9-20d4-481f-af75-8691b26a588b" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_b4b466c9-20d4-481f-af75-8691b26a588b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_1bba0197-8d38-48e7-af33-8bbb3a4658a7" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_b4b466c9-20d4-481f-af75-8691b26a588b" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_1bba0197-8d38-48e7-af33-8bbb3a4658a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LianBioMember_5ccf2f5c-a5c8-40e5-ad17-6097a48ec2c0" xlink:href="tars-20240331.xsd#tars_LianBioMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_1bba0197-8d38-48e7-af33-8bbb3a4658a7" xlink:to="loc_tars_LianBioMember_5ccf2f5c-a5c8-40e5-ad17-6097a48ec2c0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ElancoMember_ed649fda-4c67-42dd-a6ec-a50ec233dcb0" xlink:href="tars-20240331.xsd#tars_ElancoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_1bba0197-8d38-48e7-af33-8bbb3a4658a7" xlink:to="loc_tars_ElancoMember_ed649fda-4c67-42dd-a6ec-a50ec233dcb0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_GrandpharmaMember_de5f366d-d8b3-4341-ba03-a84039b95e96" xlink:href="tars-20240331.xsd#tars_GrandpharmaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_1bba0197-8d38-48e7-af33-8bbb3a4658a7" xlink:to="loc_tars_GrandpharmaMember_de5f366d-d8b3-4341-ba03-a84039b95e96" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_947a95bb-f465-4eab-8bc3-bcdfd2885885" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TypeOfArrangementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_b1c779c7-4f1c-4463-8582-d911d653adbc" xlink:to="loc_us-gaap_TypeOfArrangementAxis_947a95bb-f465-4eab-8bc3-bcdfd2885885" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_947a95bb-f465-4eab-8bc3-bcdfd2885885_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_947a95bb-f465-4eab-8bc3-bcdfd2885885" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_947a95bb-f465-4eab-8bc3-bcdfd2885885_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_14ba3b5e-08b5-424d-847c-1e102c4ca0f4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_947a95bb-f465-4eab-8bc3-bcdfd2885885" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_14ba3b5e-08b5-424d-847c-1e102c4ca0f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember_d0d2b3e1-40ff-45f2-97a1-ce03879fed23" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_14ba3b5e-08b5-424d-847c-1e102c4ca0f4" xlink:to="loc_us-gaap_CollaborativeArrangementTransactionWithPartyToCollaborativeArrangementMember_d0d2b3e1-40ff-45f2-97a1-ce03879fed23" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_149afcd6-23c6-47b5-8563-3e534ef74330" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_b1c779c7-4f1c-4463-8582-d911d653adbc" xlink:to="loc_srt_ProductOrServiceAxis_149afcd6-23c6-47b5-8563-3e534ef74330" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_149afcd6-23c6-47b5-8563-3e534ef74330_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_149afcd6-23c6-47b5-8563-3e534ef74330" xlink:to="loc_srt_ProductsAndServicesDomain_149afcd6-23c6-47b5-8563-3e534ef74330_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_cffb0464-5b1f-4cb0-a62c-ce6daeeff906" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_149afcd6-23c6-47b5-8563-3e534ef74330" xlink:to="loc_srt_ProductsAndServicesDomain_cffb0464-5b1f-4cb0-a62c-ce6daeeff906" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_MilestoneDeterminedByTheCompanyMember_540990d2-fea1-4053-99f8-32ee10fd0906" xlink:href="tars-20240331.xsd#tars_MilestoneDeterminedByTheCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cffb0464-5b1f-4cb0-a62c-ce6daeeff906" xlink:to="loc_tars_MilestoneDeterminedByTheCompanyMember_540990d2-fea1-4053-99f8-32ee10fd0906" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_DevelopmentAndRegulatoryMilestoneMember_a4325d16-4d40-4ec2-86ae-23042975e0c1" xlink:href="tars-20240331.xsd#tars_DevelopmentAndRegulatoryMilestoneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cffb0464-5b1f-4cb0-a62c-ce6daeeff906" xlink:to="loc_tars_DevelopmentAndRegulatoryMilestoneMember_a4325d16-4d40-4ec2-86ae-23042975e0c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_fbc6c635-2c93-4361-89f4-0555e0fa3889" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_b1c779c7-4f1c-4463-8582-d911d653adbc" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_fbc6c635-2c93-4361-89f4-0555e0fa3889" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_fbc6c635-2c93-4361-89f4-0555e0fa3889_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_fbc6c635-2c93-4361-89f4-0555e0fa3889" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_fbc6c635-2c93-4361-89f4-0555e0fa3889_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_fd842751-5131-4906-bdae-097125176aaa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_fbc6c635-2c93-4361-89f4-0555e0fa3889" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_fd842751-5131-4906-bdae-097125176aaa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_07d43e73-61fc-465a-9383-43a1e4aee415" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_fd842751-5131-4906-bdae-097125176aaa" xlink:to="loc_us-gaap_SubsequentEventMember_07d43e73-61fc-465a-9383-43a1e4aee415" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_93a1c5c6-d109-4576-bedd-069844fb6846" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_b1c779c7-4f1c-4463-8582-d911d653adbc" xlink:to="loc_dei_LegalEntityAxis_93a1c5c6-d109-4576-bedd-069844fb6846" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_93a1c5c6-d109-4576-bedd-069844fb6846_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_93a1c5c6-d109-4576-bedd-069844fb6846" xlink:to="loc_dei_EntityDomain_93a1c5c6-d109-4576-bedd-069844fb6846_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_4fe49de3-235d-48d8-9069-debc038f89b7" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_93a1c5c6-d109-4576-bedd-069844fb6846" xlink:to="loc_dei_EntityDomain_4fe49de3-235d-48d8-9069-debc038f89b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LianBioMember_33db2909-5e36-470b-911d-addbd855cdf3" xlink:href="tars-20240331.xsd#tars_LianBioMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_4fe49de3-235d-48d8-9069-debc038f89b7" xlink:to="loc_tars_LianBioMember_33db2909-5e36-470b-911d-addbd855cdf3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiability_f4d1ffad-12f2-4ffb-b856-e03eb91314ca" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ContractWithCustomerLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_ContractWithCustomerLiability_f4d1ffad-12f2-4ffb-b856-e03eb91314ca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_RevenueRemainingPerformanceObligationAdditionalCashPayment_f09f324c-ae8f-4529-9032-ecb086e55742" xlink:href="tars-20240331.xsd#tars_RevenueRemainingPerformanceObligationAdditionalCashPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_tars_RevenueRemainingPerformanceObligationAdditionalCashPayment_f09f324c-ae8f-4529-9032-ecb086e55742" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_RevenueRemainingPerformanceObligationVariableConsiderationAmount_784edf32-176d-4209-8fa9-71649420da18" xlink:href="tars-20240331.xsd#tars_RevenueRemainingPerformanceObligationVariableConsiderationAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_tars_RevenueRemainingPerformanceObligationVariableConsiderationAmount_784edf32-176d-4209-8fa9-71649420da18" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiCost_37599b50-78a8-486c-8628-3befad993dc9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesFvNiCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_EquitySecuritiesFvNiCost_37599b50-78a8-486c-8628-3befad993dc9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_d77dcc33-4e91-4807-b5e7-bddda6866b00" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_d77dcc33-4e91-4807-b5e7-bddda6866b00" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_TerminationPaymentPeriodDue_1e527884-03bb-4c41-8830-a815624ecd1d" xlink:href="tars-20240331.xsd#tars_TerminationPaymentPeriodDue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_tars_TerminationPaymentPeriodDue_1e527884-03bb-4c41-8830-a815624ecd1d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_7113b2f7-86e8-4b9b-9b5a-5da62b6ffe18" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_7113b2f7-86e8-4b9b-9b5a-5da62b6ffe18" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_f289e0f6-3217-4476-9848-c8dd692fdeeb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_f289e0f6-3217-4476-9848-c8dd692fdeeb" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_ProceedsFromCancellationOfWarrants_f504a64e-7647-4f54-bc06-8768e464d625" xlink:href="tars-20240331.xsd#tars_ProceedsFromCancellationOfWarrants"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_tars_ProceedsFromCancellationOfWarrants_f504a64e-7647-4f54-bc06-8768e464d625" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueIssuedForServices_2a12b545-7531-43bd-b18c-715d8e775615" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueIssuedForServices"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueIssuedForServices_2a12b545-7531-43bd-b18c-715d8e775615" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareCashPaid_1db08970-eb83-4cb7-8c05-958a4868591d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockDividendsPerShareCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_CommonStockDividendsPerShareCashPaid_1db08970-eb83-4cb7-8c05-958a4868591d" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsCommonStockCash_1b0cf267-67ab-4560-a689-223f9a2ca224" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DividendsCommonStockCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_us-gaap_DividendsCommonStockCash_1b0cf267-67ab-4560-a689-223f9a2ca224" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_UpfrontPayment_ef135890-9f47-4fc2-babb-f59a3c9ae97c" xlink:href="tars-20240331.xsd#tars_UpfrontPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_tars_UpfrontPayment_ef135890-9f47-4fc2-babb-f59a3c9ae97c" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CollaborativeArrangementMaximumMilestonePayment_218a5f2f-773b-402a-b66b-aa93d525eb93" xlink:href="tars-20240331.xsd#tars_CollaborativeArrangementMaximumMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_91e27622-f901-4448-9c7d-d6c76ff392f3" xlink:to="loc_tars_CollaborativeArrangementMaximumMilestonePayment_218a5f2f-773b-402a-b66b-aa93d525eb93" xlink:type="arc" order="13"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/CreditFacilityAgreementDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#CreditFacilityAgreementDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/CreditFacilityAgreementDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_97f8c01c-c529-426a-9288-3de65d180062" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_DebtInstrumentTable_97f8c01c-c529-426a-9288-3de65d180062" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_6ee1accb-77e6-4abf-a18a-174cd799705b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_97f8c01c-c529-426a-9288-3de65d180062" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_6ee1accb-77e6-4abf-a18a-174cd799705b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_6ee1accb-77e6-4abf-a18a-174cd799705b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_6ee1accb-77e6-4abf-a18a-174cd799705b" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_6ee1accb-77e6-4abf-a18a-174cd799705b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_ce5ac0e8-ffee-46b3-ac20-8a6418d26860" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_6ee1accb-77e6-4abf-a18a-174cd799705b" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_ce5ac0e8-ffee-46b3-ac20-8a6418d26860" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_b48082db-5feb-4cad-9400-b1466ade805f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_ce5ac0e8-ffee-46b3-ac20-8a6418d26860" xlink:to="loc_us-gaap_LineOfCreditMember_b48082db-5feb-4cad-9400-b1466ade805f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LineOfCreditSVBMember_61671e6f-0585-44ec-b1ec-a205497b14df" xlink:href="tars-20240331.xsd#tars_LineOfCreditSVBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_ce5ac0e8-ffee-46b3-ac20-8a6418d26860" xlink:to="loc_tars_LineOfCreditSVBMember_61671e6f-0585-44ec-b1ec-a205497b14df" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_5edcbc91-537d-4b30-b19f-f9a599a2cef9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_97f8c01c-c529-426a-9288-3de65d180062" xlink:to="loc_us-gaap_DebtInstrumentAxis_5edcbc91-537d-4b30-b19f-f9a599a2cef9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_5edcbc91-537d-4b30-b19f-f9a599a2cef9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_5edcbc91-537d-4b30-b19f-f9a599a2cef9" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_5edcbc91-537d-4b30-b19f-f9a599a2cef9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_9af779d0-14df-40c7-a3c0-56bc549b9d0a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_5edcbc91-537d-4b30-b19f-f9a599a2cef9" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_9af779d0-14df-40c7-a3c0-56bc549b9d0a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CreditFacilityTrancheOneMember_d7d427cc-540b-43d6-938a-fbea1b8cca5e" xlink:href="tars-20240331.xsd#tars_CreditFacilityTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9af779d0-14df-40c7-a3c0-56bc549b9d0a" xlink:to="loc_tars_CreditFacilityTrancheOneMember_d7d427cc-540b-43d6-938a-fbea1b8cca5e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CreditFacilityTrancheTwoMember_851fc337-68c8-4071-8ad0-7bfb374d0df5" xlink:href="tars-20240331.xsd#tars_CreditFacilityTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9af779d0-14df-40c7-a3c0-56bc549b9d0a" xlink:to="loc_tars_CreditFacilityTrancheTwoMember_851fc337-68c8-4071-8ad0-7bfb374d0df5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CreditFacilityTrancheThreeMember_211a4fa2-c824-4a74-a21c-2bb717101348" xlink:href="tars-20240331.xsd#tars_CreditFacilityTrancheThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9af779d0-14df-40c7-a3c0-56bc549b9d0a" xlink:to="loc_tars_CreditFacilityTrancheThreeMember_211a4fa2-c824-4a74-a21c-2bb717101348" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CreditFacilityTrancheFourMember_780f4995-307f-48d2-9944-4865dc536e90" xlink:href="tars-20240331.xsd#tars_CreditFacilityTrancheFourMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9af779d0-14df-40c7-a3c0-56bc549b9d0a" xlink:to="loc_tars_CreditFacilityTrancheFourMember_780f4995-307f-48d2-9944-4865dc536e90" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_CreditFacilityFirstAmendmentTrancheTwoMember_b0a3d504-75f6-4c35-be21-c91bb2a44822" xlink:href="tars-20240331.xsd#tars_CreditFacilityFirstAmendmentTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9af779d0-14df-40c7-a3c0-56bc549b9d0a" xlink:to="loc_tars_CreditFacilityFirstAmendmentTrancheTwoMember_b0a3d504-75f6-4c35-be21-c91bb2a44822" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_6d1eabe4-ef42-4edd-8102-5e5341b69bbe" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_97f8c01c-c529-426a-9288-3de65d180062" xlink:to="loc_us-gaap_VariableRateAxis_6d1eabe4-ef42-4edd-8102-5e5341b69bbe" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_6d1eabe4-ef42-4edd-8102-5e5341b69bbe_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_6d1eabe4-ef42-4edd-8102-5e5341b69bbe" xlink:to="loc_us-gaap_VariableRateDomain_6d1eabe4-ef42-4edd-8102-5e5341b69bbe_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_0f13a6cf-eda4-4a5c-8011-16c115ba83d2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_6d1eabe4-ef42-4edd-8102-5e5341b69bbe" xlink:to="loc_us-gaap_VariableRateDomain_0f13a6cf-eda4-4a5c-8011-16c115ba83d2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrimeRateMember_dd713d54-3802-4921-8f53-e48e066bef04" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PrimeRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_0f13a6cf-eda4-4a5c-8011-16c115ba83d2" xlink:to="loc_us-gaap_PrimeRateMember_dd713d54-3802-4921-8f53-e48e066bef04" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_0a4f2c0d-5e98-4949-bb8f-53c54644c28d" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_97f8c01c-c529-426a-9288-3de65d180062" xlink:to="loc_srt_RangeAxis_0a4f2c0d-5e98-4949-bb8f-53c54644c28d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_0a4f2c0d-5e98-4949-bb8f-53c54644c28d_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_0a4f2c0d-5e98-4949-bb8f-53c54644c28d" xlink:to="loc_srt_RangeMember_0a4f2c0d-5e98-4949-bb8f-53c54644c28d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ab088684-f6cc-40f9-8bad-5db14fda1712" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_0a4f2c0d-5e98-4949-bb8f-53c54644c28d" xlink:to="loc_srt_RangeMember_ab088684-f6cc-40f9-8bad-5db14fda1712" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_f80db418-bb48-4af6-ad20-0c4e71176cc1" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ab088684-f6cc-40f9-8bad-5db14fda1712" xlink:to="loc_srt_MaximumMember_f80db418-bb48-4af6-ad20-0c4e71176cc1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromLinesOfCredit_7401b8d0-bfa5-435e-9475-0dcbef530235" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromLinesOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_ProceedsFromLinesOfCredit_7401b8d0-bfa5-435e-9475-0dcbef530235" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_0d5a0c7b-4014-4728-9d71-c34057c431e2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_0d5a0c7b-4014-4728-9d71-c34057c431e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LineOfCreditFacilityDrawDownIncrements_c3514d38-a40b-4ee4-974d-5678b5926bcc" xlink:href="tars-20240331.xsd#tars_LineOfCreditFacilityDrawDownIncrements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_tars_LineOfCreditFacilityDrawDownIncrements_c3514d38-a40b-4ee4-974d-5678b5926bcc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_a7ee938f-0d72-4d6e-912e-32894d2c54c4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_a7ee938f-0d72-4d6e-912e-32894d2c54c4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_179a7b81-4a95-40c5-b6dc-7d711954a1cd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_179a7b81-4a95-40c5-b6dc-7d711954a1cd" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityInterestRateDuringPeriod_2638f136-e062-4ba1-8d16-bcb1a8c421a6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityInterestRateDuringPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_LineOfCreditFacilityInterestRateDuringPeriod_2638f136-e062-4ba1-8d16-bcb1a8c421a6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityPeriodicPaymentInterest_e22f25d0-b3d8-4d79-96bd-baa41e6b21d6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityPeriodicPaymentInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_LineOfCreditFacilityPeriodicPaymentInterest_e22f25d0-b3d8-4d79-96bd-baa41e6b21d6" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_LineOfCreditFacilityPeriodicPaymentInterestRate_ea19f36f-aae3-473e-9e2a-eed4e4148115" xlink:href="tars-20240331.xsd#tars_LineOfCreditFacilityPeriodicPaymentInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_tars_LineOfCreditFacilityPeriodicPaymentInterestRate_ea19f36f-aae3-473e-9e2a-eed4e4148115" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_0460e500-13c5-43d6-bdd9-30c17c3de49d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_0460e500-13c5-43d6-bdd9-30c17c3de49d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_2c835e7a-dfd5-489c-86c3-195a58f54253" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_63c89ab8-6c94-4d5d-a19a-3676f62acc83" xlink:to="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_2c835e7a-dfd5-489c-86c3-195a58f54253" xlink:type="arc" order="9"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/RelatedPartyTransactionsDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#RelatedPartyTransactionsDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/RelatedPartyTransactionsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_d4fa28f8-99b7-4477-a80c-7be7f29bcb52" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_d460d98c-4acf-44e1-adf5-98bc5c824bb2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_d4fa28f8-99b7-4477-a80c-7be7f29bcb52" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_d460d98c-4acf-44e1-adf5-98bc5c824bb2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f45310c0-d454-4877-9aec-af788c9aac6b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_d460d98c-4acf-44e1-adf5-98bc5c824bb2" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f45310c0-d454-4877-9aec-af788c9aac6b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_f45310c0-d454-4877-9aec-af788c9aac6b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f45310c0-d454-4877-9aec-af788c9aac6b" xlink:to="loc_us-gaap_RelatedPartyDomain_f45310c0-d454-4877-9aec-af788c9aac6b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_a10914e0-2e60-447a-8ab0-99a0e9cdd1c0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f45310c0-d454-4877-9aec-af788c9aac6b" xlink:to="loc_us-gaap_RelatedPartyDomain_a10914e0-2e60-447a-8ab0-99a0e9cdd1c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_DirectorMember_50112458-7dd0-4e24-ba6b-76af7c395716" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_DirectorMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_a10914e0-2e60-447a-8ab0-99a0e9cdd1c0" xlink:to="loc_srt_DirectorMember_50112458-7dd0-4e24-ba6b-76af7c395716" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyMember_189b1570-7e1b-4f4b-b3a3-2c577ca44c8e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_a10914e0-2e60-447a-8ab0-99a0e9cdd1c0" xlink:to="loc_us-gaap_RelatedPartyMember_189b1570-7e1b-4f4b-b3a3-2c577ca44c8e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_2f9210f6-ab15-4ddb-a8c8-29cc3453a7af" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_d460d98c-4acf-44e1-adf5-98bc5c824bb2" xlink:to="loc_srt_RangeAxis_2f9210f6-ab15-4ddb-a8c8-29cc3453a7af" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_2f9210f6-ab15-4ddb-a8c8-29cc3453a7af_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_2f9210f6-ab15-4ddb-a8c8-29cc3453a7af" xlink:to="loc_srt_RangeMember_2f9210f6-ab15-4ddb-a8c8-29cc3453a7af_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_1fffa57c-c2b0-4929-88d3-2d00995d8b84" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_2f9210f6-ab15-4ddb-a8c8-29cc3453a7af" xlink:to="loc_srt_RangeMember_1fffa57c-c2b0-4929-88d3-2d00995d8b84" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_acfe5593-4bfe-4875-8c0f-2682ef4a597e" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_1fffa57c-c2b0-4929-88d3-2d00995d8b84" xlink:to="loc_srt_MinimumMember_acfe5593-4bfe-4875-8c0f-2682ef4a597e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_45b2e140-3c33-4165-a8c1-56b696287b25" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_1fffa57c-c2b0-4929-88d3-2d00995d8b84" xlink:to="loc_srt_MaximumMember_45b2e140-3c33-4165-a8c1-56b696287b25" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_RelatedPartyTransactionAnnualCashCompensation_d910e2fd-dca7-4f7b-8c5a-4e31add989c5" xlink:href="tars-20240331.xsd#tars_RelatedPartyTransactionAnnualCashCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_d4fa28f8-99b7-4477-a80c-7be7f29bcb52" xlink:to="loc_tars_RelatedPartyTransactionAnnualCashCompensation_d910e2fd-dca7-4f7b-8c5a-4e31add989c5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_9ab0ba24-e0f1-432f-9a61-a48e789253d0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_d4fa28f8-99b7-4477-a80c-7be7f29bcb52" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_9ab0ba24-e0f1-432f-9a61-a48e789253d0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_1e89516d-7451-4552-bbdd-4beab07a1a9c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_d4fa28f8-99b7-4477-a80c-7be7f29bcb52" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_1e89516d-7451-4552-bbdd-4beab07a1a9c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_RelatedPartTransactionTerminationNoticePeriod_8f569c34-4a43-444c-bb9d-05ebe847a9e8" xlink:href="tars-20240331.xsd#tars_RelatedPartTransactionTerminationNoticePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_d4fa28f8-99b7-4477-a80c-7be7f29bcb52" xlink:to="loc_tars_RelatedPartTransactionTerminationNoticePeriod_8f569c34-4a43-444c-bb9d-05ebe847a9e8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_e45cafa1-fda1-49ab-af0f-2756cbe80a20" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_d4fa28f8-99b7-4477-a80c-7be7f29bcb52" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_e45cafa1-fda1-49ab-af0f-2756cbe80a20" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://tarsusrx.com/role/SubsequentEventDetails" xlink:type="simple" xlink:href="tars-20240331.xsd#SubsequentEventDetails"/>
  <link:definitionLink xlink:role="http://tarsusrx.com/role/SubsequentEventDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTable_14ef90e7-b712-4054-96d0-1aac51bde9aa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_us-gaap_SubsequentEventTable_14ef90e7-b712-4054-96d0-1aac51bde9aa" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_5ca565ed-9e37-4f05-9ca6-36d0f4b30742" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_14ef90e7-b712-4054-96d0-1aac51bde9aa" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_5ca565ed-9e37-4f05-9ca6-36d0f4b30742" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_5ca565ed-9e37-4f05-9ca6-36d0f4b30742_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_5ca565ed-9e37-4f05-9ca6-36d0f4b30742" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_5ca565ed-9e37-4f05-9ca6-36d0f4b30742_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_54134e9b-d2a1-4d87-8936-de5b30a0c070" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_5ca565ed-9e37-4f05-9ca6-36d0f4b30742" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_54134e9b-d2a1-4d87-8936-de5b30a0c070" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_aee5eeba-bd52-4787-8a70-3a822376ad2e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_54134e9b-d2a1-4d87-8936-de5b30a0c070" xlink:to="loc_us-gaap_SubsequentEventMember_aee5eeba-bd52-4787-8a70-3a822376ad2e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_b380e7c1-4f59-40ad-beaa-25c7edf51155" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_14ef90e7-b712-4054-96d0-1aac51bde9aa" xlink:to="loc_us-gaap_DebtInstrumentAxis_b380e7c1-4f59-40ad-beaa-25c7edf51155" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_b380e7c1-4f59-40ad-beaa-25c7edf51155_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_b380e7c1-4f59-40ad-beaa-25c7edf51155" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_b380e7c1-4f59-40ad-beaa-25c7edf51155_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_285fea64-c19c-4094-84c1-955e983feba5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_b380e7c1-4f59-40ad-beaa-25c7edf51155" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_285fea64-c19c-4094-84c1-955e983feba5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_PharmakonCreditFacilityMember_47053513-c9f9-4bb2-879f-3df7234645e0" xlink:href="tars-20240331.xsd#tars_PharmakonCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_285fea64-c19c-4094-84c1-955e983feba5" xlink:to="loc_tars_PharmakonCreditFacilityMember_47053513-c9f9-4bb2-879f-3df7234645e0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_PharmakonCreditFacilityTrancheOneMember_6aada130-5e33-4464-9117-f32e0d49c114" xlink:href="tars-20240331.xsd#tars_PharmakonCreditFacilityTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_tars_PharmakonCreditFacilityMember_47053513-c9f9-4bb2-879f-3df7234645e0" xlink:to="loc_tars_PharmakonCreditFacilityTrancheOneMember_6aada130-5e33-4464-9117-f32e0d49c114" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_PharmakonCreditFacilityTrancheTwoMember_8705f744-2f75-45a8-a1c7-a11b360b0ab4" xlink:href="tars-20240331.xsd#tars_PharmakonCreditFacilityTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_tars_PharmakonCreditFacilityMember_47053513-c9f9-4bb2-879f-3df7234645e0" xlink:to="loc_tars_PharmakonCreditFacilityTrancheTwoMember_8705f744-2f75-45a8-a1c7-a11b360b0ab4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_PharmakonCreditFacilityTrancheThreeMember_04c39a69-836f-4be8-950c-a14f324a57e1" xlink:href="tars-20240331.xsd#tars_PharmakonCreditFacilityTrancheThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_tars_PharmakonCreditFacilityMember_47053513-c9f9-4bb2-879f-3df7234645e0" xlink:to="loc_tars_PharmakonCreditFacilityTrancheThreeMember_04c39a69-836f-4be8-950c-a14f324a57e1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_PharmakonCreditFacilityTrancheFourMember_722c533e-c9e7-4723-a2f7-de3b43ced3c6" xlink:href="tars-20240331.xsd#tars_PharmakonCreditFacilityTrancheFourMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_tars_PharmakonCreditFacilityMember_47053513-c9f9-4bb2-879f-3df7234645e0" xlink:to="loc_tars_PharmakonCreditFacilityTrancheFourMember_722c533e-c9e7-4723-a2f7-de3b43ced3c6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_49743f69-a4ee-43af-bb3d-cfeda18ad84f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_14ef90e7-b712-4054-96d0-1aac51bde9aa" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_49743f69-a4ee-43af-bb3d-cfeda18ad84f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_49743f69-a4ee-43af-bb3d-cfeda18ad84f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_49743f69-a4ee-43af-bb3d-cfeda18ad84f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_49743f69-a4ee-43af-bb3d-cfeda18ad84f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_8a51d35e-7bd5-411f-ba41-1bb2b4e954dc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_49743f69-a4ee-43af-bb3d-cfeda18ad84f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_8a51d35e-7bd5-411f-ba41-1bb2b4e954dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_6d7a7f59-ec8a-4056-a1dd-b0ec3f40b8c8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_8a51d35e-7bd5-411f-ba41-1bb2b4e954dc" xlink:to="loc_us-gaap_LineOfCreditMember_6d7a7f59-ec8a-4056-a1dd-b0ec3f40b8c8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_bd9962d5-e0b3-4cbe-8e51-06da2fbe3ea6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_14ef90e7-b712-4054-96d0-1aac51bde9aa" xlink:to="loc_us-gaap_VariableRateAxis_bd9962d5-e0b3-4cbe-8e51-06da2fbe3ea6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_bd9962d5-e0b3-4cbe-8e51-06da2fbe3ea6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_bd9962d5-e0b3-4cbe-8e51-06da2fbe3ea6" xlink:to="loc_us-gaap_VariableRateDomain_bd9962d5-e0b3-4cbe-8e51-06da2fbe3ea6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_ec4d5b17-331b-4565-a0e6-8c54f473939b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_bd9962d5-e0b3-4cbe-8e51-06da2fbe3ea6" xlink:to="loc_us-gaap_VariableRateDomain_ec4d5b17-331b-4565-a0e6-8c54f473939b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_80f19217-42ba-498f-825f-737df6531b41" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_ec4d5b17-331b-4565-a0e6-8c54f473939b" xlink:to="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_80f19217-42ba-498f-825f-737df6531b41" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromLinesOfCredit_ec9a737f-e99a-4230-b821-df3cb88e3c00" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromLinesOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_us-gaap_ProceedsFromLinesOfCredit_ec9a737f-e99a-4230-b821-df3cb88e3c00" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromRepaymentsOfDebt_cbbfd739-9143-428a-94d7-e221309f5bd4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromRepaymentsOfDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_us-gaap_ProceedsFromRepaymentsOfDebt_cbbfd739-9143-428a-94d7-e221309f5bd4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_DebtInstrumentNumberOfPotentialAdditionalTranches_9ddb7f3b-3841-4941-b259-efaa7c943197" xlink:href="tars-20240331.xsd#tars_DebtInstrumentNumberOfPotentialAdditionalTranches"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_tars_DebtInstrumentNumberOfPotentialAdditionalTranches_9ddb7f3b-3841-4941-b259-efaa7c943197" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_e170842a-fede-4cda-bcb3-98fa8596480d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_e170842a-fede-4cda-bcb3-98fa8596480d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_DebtInstrumentNumberOfTranchesWithMilestoneRequirements_b75bdbcb-3c6b-4e01-a2aa-410f9ab7a4b9" xlink:href="tars-20240331.xsd#tars_DebtInstrumentNumberOfTranchesWithMilestoneRequirements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_tars_DebtInstrumentNumberOfTranchesWithMilestoneRequirements_b75bdbcb-3c6b-4e01-a2aa-410f9ab7a4b9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_2722ab0b-b47e-47e2-9247-29f8442873fc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_2722ab0b-b47e-47e2-9247-29f8442873fc" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_DebtInstrumentFloorInterestRate_f61ebb37-e277-4e54-af26-eda7df02f1aa" xlink:href="tars-20240331.xsd#tars_DebtInstrumentFloorInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_tars_DebtInstrumentFloorInterestRate_f61ebb37-e277-4e54-af26-eda7df02f1aa" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireEquitySecuritiesFvNi_d4d57396-0452-4ae1-bd4c-a71685969dbc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PaymentsToAcquireEquitySecuritiesFvNi"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5a45e30a-fd32-4ad2-b82b-72baffdafed7" xlink:to="loc_us-gaap_PaymentsToAcquireEquitySecuritiesFvNi_d4d57396-0452-4ae1-bd4c-a71685969dbc" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://xbrl.sec.gov/ecd/AwardTypeOnly" xlink:type="simple" xlink:href="https://xbrl.sec.gov/ecd/2023/ecd-2023.xsd#AwardTypeOnly"/>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/ecd/AwardTypeOnly" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_us-gaap_EmployeeStockMember" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:type="simple" xlink:href="https://xbrl.sec.gov/ecd/2023/ecd-2023.xsd#IndividualsOnly"/>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ecd_AllIndividualsMember" xlink:href="https://xbrl.sec.gov/ecd/2023/ecd-2023.xsd#ecd_AllIndividualsMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_BobakAzamianMember_bb5096da-9df8-437c-949c-0e12b46b0cb2" xlink:href="tars-20240331.xsd#tars_BobakAzamianMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ecd_AllIndividualsMember" xlink:to="loc_tars_BobakAzamianMember_bb5096da-9df8-437c-949c-0e12b46b0cb2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tars_JoseTrevejoMember_bfb3b605-365e-4bf6-872f-687eb0bfda09" xlink:href="tars-20240331.xsd#tars_JoseTrevejoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ecd_AllIndividualsMember" xlink:to="loc_tars_JoseTrevejoMember_bfb3b605-365e-4bf6-872f-687eb0bfda09" xlink:type="arc" order="3"/>
  </link:definitionLink>
</link:linkbase>
